Analysis of Novel 5'-UTR Polyadenylation Sites in Arabidopsis thaliana

Analysis of Novel 5'-UTR Polyadenylation Sites in Arabidopsis thaliana

Bibliographic Details
Main Author: Yingdong, Zhu
Language:English
Published: Miami University / OhioLINK 2016
Subjects:
Biology
5-UTR
alternative polyadenylation
open reading frame
Online Access:http://rave.ohiolink.edu/etdc/view?acc_num=miami1480717686996059
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spelling ndltd-OhioLink-oai-etd.ohiolink.edu-miami14807176869960592021-08-03T06:39:22Z Analysis of Novel 5'-UTR Polyadenylation Sites in Arabidopsis thaliana Yingdong, Zhu Biology 5-UTR alternative polyadenylation open reading frame Messenger RNA (mRNA) polyadenylation is an indispensable step during post-transcriptional pre-mRNA processing in eukaryotes. The usage of one poly(A) site over another is known as alternative polyadenylation (APA). APA has been implicated in gene expression regulation through its role of selecting the ends of a transcript. Recent studies of polyadenylation profiles in the Arabidopsis database predicted that a portion of the poly(A) sites are located in the 5'-UTR. Here, we first describe 5'-UTR-APA and verify existence of independent polyadenylated 5'-UTR transcripts, arising due to alternative polyadenylation in the 5'-UTRs of the corresponding genes. We used 3'-RACE and sequencing to validate poly(A) sites and Northern blot to show that the observed short upstream transcripts do not arise from the 3'-end of a previously unrecognized convergent gene. We hypothesize that 5'-UTR polyadenylation may represent a gene silencing mechanism, and that truncated 5'-UTR transcripts may have functions, either existing as polyadenylated non-coding RNAs, or potentially to regulate gene expression by encoding a short peptide, which we call a 5'-UTR ORF. We propose a model to explain the features and functions of 5'-UTR ORF as well as the relationships between upstream ORF (uORF) and major ORF (mORF). 2016-12-06 English text Miami University / OhioLINK http://rave.ohiolink.edu/etdc/view?acc_num=miami1480717686996059 http://rave.ohiolink.edu/etdc/view?acc_num=miami1480717686996059 unrestricted This thesis or dissertation is protected by copyright: all rights reserved. It may not be copied or redistributed beyond the terms of applicable copyright laws.
collection NDLTD
language English
sources NDLTD
topic Biology
5-UTR
alternative polyadenylation
open reading frame
spellingShingle Biology
5-UTR
alternative polyadenylation
open reading frame
Yingdong, Zhu
Analysis of Novel 5'-UTR Polyadenylation Sites in Arabidopsis thaliana
author Yingdong, Zhu
author_facet Yingdong, Zhu
author_sort Yingdong, Zhu
title Analysis of Novel 5'-UTR Polyadenylation Sites in Arabidopsis thaliana
title_short Analysis of Novel 5'-UTR Polyadenylation Sites in Arabidopsis thaliana
title_full Analysis of Novel 5'-UTR Polyadenylation Sites in Arabidopsis thaliana
title_fullStr Analysis of Novel 5'-UTR Polyadenylation Sites in Arabidopsis thaliana
title_full_unstemmed Analysis of Novel 5'-UTR Polyadenylation Sites in Arabidopsis thaliana
title_sort analysis of novel 5'-utr polyadenylation sites in arabidopsis thaliana
publisher Miami University / OhioLINK
publishDate 2016
url http://rave.ohiolink.edu/etdc/view?acc_num=miami1480717686996059
work_keys_str_mv AT yingdongzhu analysisofnovel5utrpolyadenylationsitesinarabidopsisthaliana
_version_ 1719440943276359680

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