Nitric oxide metabolites in hypoxia, freezing, and hibernation of the wood frog, <i>Rana sylvatica</i>

Bibliographic Details
Main Author: Bethany, Williams
Language:English
Published: Miami University / OhioLINK 2018
Subjects:
Online Access:http://rave.ohiolink.edu/etdc/view?acc_num=miami1525202133341325
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spelling ndltd-OhioLink-oai-etd.ohiolink.edu-miami15252021333413252021-08-03T07:06:36Z Nitric oxide metabolites in hypoxia, freezing, and hibernation of the wood frog, <i>Rana sylvatica</i> Bethany, Williams Biology Physiology freezing hibernation rana sylvatica nitric oxide hypoxia Nitric oxide (NO) is a gaseous free radical that in diverse organisms performs many signaling and protective functions, including: vasodilation, inhibition of apoptosis, antioxidation, and metabolic downregulation. Increased NO availability may be especially important during periods when organisms must contend with multiple stresses. We investigated stress-induced changes in NO metabolites, nitrite (NO<sub>2</sub><sup>-</sup>) and nitrate (NO<sub>3</sub><sup>-</sup>), in the blood plasma, heart, liver, and gracilis of the wood frog (<i>Rana sylvatica</i>), which endures chronic cold, freezing, hypoxia, dehydration, and extended aphagia during hibernation. We found elevated concentrations of NO<sub>2</sub><sup>-</sup> and/or NO<sub>3</sub><sup>-</sup> in the plasma, heart, and liver of hypoxia-exposed frogs (24 h, 4 °C), and in liver of frogs exposed to freezing (48 h, -2.5 °C), suggesting that increased NO availability aids in surviving these stresses. During a 32-week period of simulated hibernation, NO<sub>2</sub><sup>-</sup> and/or NO<sub>3</sub><sup>-</sup> increased in the plasma, heart, and liver during an initial 5-week winter-conditioning regimen and generally remained elevated thereafter. In hibernation, plasma NO<sub>2</sub><sup>-</sup> was higher in frogs indigenous to Interior Alaska than in conspecifics from a temperate locale. Our results, together with published values for other species, suggest that the NO protection system is of general importance in the stress adaptation of vertebrates. 2018-05-02 English text Miami University / OhioLINK http://rave.ohiolink.edu/etdc/view?acc_num=miami1525202133341325 http://rave.ohiolink.edu/etdc/view?acc_num=miami1525202133341325 unrestricted This thesis or dissertation is protected by copyright: all rights reserved. It may not be copied or redistributed beyond the terms of applicable copyright laws.
collection NDLTD
language English
sources NDLTD
topic Biology
Physiology
freezing
hibernation
rana sylvatica
nitric oxide
hypoxia
spellingShingle Biology
Physiology
freezing
hibernation
rana sylvatica
nitric oxide
hypoxia
Bethany, Williams
Nitric oxide metabolites in hypoxia, freezing, and hibernation of the wood frog, <i>Rana sylvatica</i>
author Bethany, Williams
author_facet Bethany, Williams
author_sort Bethany, Williams
title Nitric oxide metabolites in hypoxia, freezing, and hibernation of the wood frog, <i>Rana sylvatica</i>
title_short Nitric oxide metabolites in hypoxia, freezing, and hibernation of the wood frog, <i>Rana sylvatica</i>
title_full Nitric oxide metabolites in hypoxia, freezing, and hibernation of the wood frog, <i>Rana sylvatica</i>
title_fullStr Nitric oxide metabolites in hypoxia, freezing, and hibernation of the wood frog, <i>Rana sylvatica</i>
title_full_unstemmed Nitric oxide metabolites in hypoxia, freezing, and hibernation of the wood frog, <i>Rana sylvatica</i>
title_sort nitric oxide metabolites in hypoxia, freezing, and hibernation of the wood frog, <i>rana sylvatica</i>
publisher Miami University / OhioLINK
publishDate 2018
url http://rave.ohiolink.edu/etdc/view?acc_num=miami1525202133341325
work_keys_str_mv AT bethanywilliams nitricoxidemetabolitesinhypoxiafreezingandhibernationofthewoodfrogiranasylvaticai
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