Nitric oxide metabolites in hypoxia, freezing, and hibernation of the wood frog, <i>Rana sylvatica</i>
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ndltd-OhioLink-oai-etd.ohiolink.edu-miami15252021333413252021-08-03T07:06:36Z Nitric oxide metabolites in hypoxia, freezing, and hibernation of the wood frog, <i>Rana sylvatica</i> Bethany, Williams Biology Physiology freezing hibernation rana sylvatica nitric oxide hypoxia Nitric oxide (NO) is a gaseous free radical that in diverse organisms performs many signaling and protective functions, including: vasodilation, inhibition of apoptosis, antioxidation, and metabolic downregulation. Increased NO availability may be especially important during periods when organisms must contend with multiple stresses. We investigated stress-induced changes in NO metabolites, nitrite (NO<sub>2</sub><sup>-</sup>) and nitrate (NO<sub>3</sub><sup>-</sup>), in the blood plasma, heart, liver, and gracilis of the wood frog (<i>Rana sylvatica</i>), which endures chronic cold, freezing, hypoxia, dehydration, and extended aphagia during hibernation. We found elevated concentrations of NO<sub>2</sub><sup>-</sup> and/or NO<sub>3</sub><sup>-</sup> in the plasma, heart, and liver of hypoxia-exposed frogs (24 h, 4 °C), and in liver of frogs exposed to freezing (48 h, -2.5 °C), suggesting that increased NO availability aids in surviving these stresses. During a 32-week period of simulated hibernation, NO<sub>2</sub><sup>-</sup> and/or NO<sub>3</sub><sup>-</sup> increased in the plasma, heart, and liver during an initial 5-week winter-conditioning regimen and generally remained elevated thereafter. In hibernation, plasma NO<sub>2</sub><sup>-</sup> was higher in frogs indigenous to Interior Alaska than in conspecifics from a temperate locale. Our results, together with published values for other species, suggest that the NO protection system is of general importance in the stress adaptation of vertebrates. 2018-05-02 English text Miami University / OhioLINK http://rave.ohiolink.edu/etdc/view?acc_num=miami1525202133341325 http://rave.ohiolink.edu/etdc/view?acc_num=miami1525202133341325 unrestricted This thesis or dissertation is protected by copyright: all rights reserved. It may not be copied or redistributed beyond the terms of applicable copyright laws. |
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English |
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Biology Physiology freezing hibernation rana sylvatica nitric oxide hypoxia |
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Biology Physiology freezing hibernation rana sylvatica nitric oxide hypoxia Bethany, Williams Nitric oxide metabolites in hypoxia, freezing, and hibernation of the wood frog, <i>Rana sylvatica</i> |
author |
Bethany, Williams |
author_facet |
Bethany, Williams |
author_sort |
Bethany, Williams |
title |
Nitric oxide metabolites in hypoxia, freezing, and hibernation of the wood frog, <i>Rana sylvatica</i> |
title_short |
Nitric oxide metabolites in hypoxia, freezing, and hibernation of the wood frog, <i>Rana sylvatica</i> |
title_full |
Nitric oxide metabolites in hypoxia, freezing, and hibernation of the wood frog, <i>Rana sylvatica</i> |
title_fullStr |
Nitric oxide metabolites in hypoxia, freezing, and hibernation of the wood frog, <i>Rana sylvatica</i> |
title_full_unstemmed |
Nitric oxide metabolites in hypoxia, freezing, and hibernation of the wood frog, <i>Rana sylvatica</i> |
title_sort |
nitric oxide metabolites in hypoxia, freezing, and hibernation of the wood frog, <i>rana sylvatica</i> |
publisher |
Miami University / OhioLINK |
publishDate |
2018 |
url |
http://rave.ohiolink.edu/etdc/view?acc_num=miami1525202133341325 |
work_keys_str_mv |
AT bethanywilliams nitricoxidemetabolitesinhypoxiafreezingandhibernationofthewoodfrogiranasylvaticai |
_version_ |
1719453972137246720 |