In vivo and in vitro rapid cold-hardening in the Antarctic midge, Belgica antarctica: Evidence of a role for calcium
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Miami University Honors Theses / OhioLINK
2007
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ndltd-OhioLink-oai-etd.ohiolink.edu-muhonors11779562942021-08-03T05:42:16Z In vivo and in vitro rapid cold-hardening in the Antarctic midge, Belgica antarctica: Evidence of a role for calcium Teets, Nick M. rapid cold-hardening cryobiology Antarctica calcium physiological ecology insect physiology In many invertebrates, the rapid cold-hardening (RCH) response protects against chilling injury at both the organismal and cellular level. The Antarctic midge,Belgica antarctica, exhibits a novel form of RCH, by which it increases its freezing tolerance. In this study, we examined 1) the effect of in vivo RCH on organismal and cell survival, 2) whether RCH in B. antarctica can occur in isolated tissues in vitro, 3) whether the freeze-tolerance of tissues depends on whether RCH occurs in the supercooled or frozen state, and 4) whether calcium is required for RCH to occur in isolated tissues. One hour of exposure at -5°C significantly increased organismal freezing tolerance at both -15°C and -20°C. Similarly, RCH significantly increased the cell survival of fat body, Malpighian tubules, and gut tissue of larvae frozen at -20°C. Furthermore, isolated tissues retained the capacity for RCH, as brief exposure to -5°C significantly increased the cell survival of isolated Malpighian tubules and gut tissue frozen at -20°C. This indicates that RCH can enhance freezing tolerance at the cellular level without neuroendocrine input. Interestingly, there was no difference in survival between tissues supercooled at -5°C and those frozen at -5°C during RCH, suggesting that temperature mediates RCH independent of the freezing of body fluids. Finally, we found that calcium is required for RCH to occur. Removing calcium from the incubating solution had a slight effect on cell survival in the RCH treatments, while blocking calcium with the intracellular chelator BAPTA-AM caused a significant reduction in survival in the RCH treatments only. Therefore, it appears that calcium is an important second messenger involved in the sensing and transduction of the RCH response. 2007-05-02 English text Miami University Honors Theses / OhioLINK http://rave.ohiolink.edu/etdc/view?acc_num=muhonors1177956294 http://rave.ohiolink.edu/etdc/view?acc_num=muhonors1177956294 unrestricted This thesis or dissertation is protected by copyright: all rights reserved. It may not be copied or redistributed beyond the terms of applicable copyright laws. |
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NDLTD |
| language |
English |
| sources |
NDLTD |
| topic |
rapid cold-hardening cryobiology Antarctica calcium physiological ecology insect physiology |
| spellingShingle |
rapid cold-hardening cryobiology Antarctica calcium physiological ecology insect physiology Teets, Nick M. In vivo and in vitro rapid cold-hardening in the Antarctic midge, Belgica antarctica: Evidence of a role for calcium |
| author |
Teets, Nick M. |
| author_facet |
Teets, Nick M. |
| author_sort |
Teets, Nick M. |
| title |
In vivo and in vitro rapid cold-hardening in the Antarctic midge, Belgica antarctica: Evidence of a role for calcium |
| title_short |
In vivo and in vitro rapid cold-hardening in the Antarctic midge, Belgica antarctica: Evidence of a role for calcium |
| title_full |
In vivo and in vitro rapid cold-hardening in the Antarctic midge, Belgica antarctica: Evidence of a role for calcium |
| title_fullStr |
In vivo and in vitro rapid cold-hardening in the Antarctic midge, Belgica antarctica: Evidence of a role for calcium |
| title_full_unstemmed |
In vivo and in vitro rapid cold-hardening in the Antarctic midge, Belgica antarctica: Evidence of a role for calcium |
| title_sort |
in vivo and in vitro rapid cold-hardening in the antarctic midge, belgica antarctica: evidence of a role for calcium |
| publisher |
Miami University Honors Theses / OhioLINK |
| publishDate |
2007 |
| url |
http://rave.ohiolink.edu/etdc/view?acc_num=muhonors1177956294 |
| work_keys_str_mv |
AT teetsnickm invivoandinvitrorapidcoldhardeningintheantarcticmidgebelgicaantarcticaevidenceofaroleforcalcium |
| _version_ |
1719423823896379392 |