The Role of Calpains in UVB-Induced Inhibitor Kappa B Alpha Degradation

The Role of Calpains in UVB-Induced Inhibitor Kappa B Alpha Degradation

Bibliographic Details
Main Author: Morris, Rachel A.
Language:English
Published: Ohio University / OhioLINK 2012
Subjects:
Biochemistry
Molecular Biology
UVB
calpain
calpeptin
IkBa
CK2
skin cancer
Online Access:http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1337887851
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spelling ndltd-OhioLink-oai-etd.ohiolink.edu-ohiou13378878512021-08-03T05:47:17Z The Role of Calpains in UVB-Induced Inhibitor Kappa B Alpha Degradation Morris, Rachel A. Biochemistry Molecular Biology UVB calpain calpeptin IkBa CK2 skin cancer Skin cancer is the most prevalent cancer in the United States and is usually induced by UV exposure. UV radiation is known to activate both calpains and NF-¿¿¿¿B, two proteins whose activity has been implicated in cancer development and progression. The inhibitor of ¿¿¿¿B (I¿¿¿¿B) is a short half-life protein that is affected by UV-induced translation inhibition and additionally can be a substrate of calpain. This project seeks to elucidate a correlation between calpain, I¿¿¿¿B¿¿¿¿, translation inhibition, and cell viability upon UVB-irradiation, utilizing two cell lines with three key mutants, three inhibitors, flow cytometry, and Western blotting. Results show that calpain does not actively degrade I¿¿¿¿B¿¿¿¿ upon UVB irradiation, but that translation inhibition alone cannot account for decreased I¿¿¿¿B¿¿¿¿ levels. Furthermore, in MEF cells, calpain inhibition was found to induce translation inhibition and increase cell viability after UVB irradiation. 2012-07-26 English text Ohio University / OhioLINK http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1337887851 http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1337887851 unrestricted This thesis or dissertation is protected by copyright: all rights reserved. It may not be copied or redistributed beyond the terms of applicable copyright laws.
collection NDLTD
language English
sources NDLTD
topic Biochemistry
Molecular Biology
UVB
calpain
calpeptin
IkBa
CK2
skin cancer
spellingShingle Biochemistry
Molecular Biology
UVB
calpain
calpeptin
IkBa
CK2
skin cancer
Morris, Rachel A.
The Role of Calpains in UVB-Induced Inhibitor Kappa B Alpha Degradation
author Morris, Rachel A.
author_facet Morris, Rachel A.
author_sort Morris, Rachel A.
title The Role of Calpains in UVB-Induced Inhibitor Kappa B Alpha Degradation
title_short The Role of Calpains in UVB-Induced Inhibitor Kappa B Alpha Degradation
title_full The Role of Calpains in UVB-Induced Inhibitor Kappa B Alpha Degradation
title_fullStr The Role of Calpains in UVB-Induced Inhibitor Kappa B Alpha Degradation
title_full_unstemmed The Role of Calpains in UVB-Induced Inhibitor Kappa B Alpha Degradation
title_sort role of calpains in uvb-induced inhibitor kappa b alpha degradation
publisher Ohio University / OhioLINK
publishDate 2012
url http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1337887851
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