Effect of Different Light Intensities on Freshwater Red Algae Batrachospermum gelatinosum: A Transcriptomic Approach

Effect of Different Light Intensities on Freshwater Red Algae Batrachospermum gelatinosum: A Transcriptomic Approach

Bibliographic Details
Main Author: Tiwari, Sunil
Language:English
Published: Ohio University / OhioLINK 2020
Subjects:
Plant Biology
Photoinhibition
Photoprotection
Photoadaptation
Online Access:http://rave.ohiolink.edu/etdc/view?acc_num=ohiou159671675380422
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spelling ndltd-OhioLink-oai-etd.ohiolink.edu-ohiou1596716753804222021-09-02T05:10:39Z Effect of Different Light Intensities on Freshwater Red Algae Batrachospermum gelatinosum: A Transcriptomic Approach Tiwari, Sunil Plant Biology Photoinhibition Photoprotection Photoadaptation Freshwater red algae are common primary producers in streams and rivers throughout the world. Physiological studies of these algae have shown that they are shade-adapted and can acclimate to various light conditions. Only two studies on gene regulation have been conducted in freshwater red algae to elucidate the effect of light on photosynthesis. Batrachospermum gelatinosum commonly inhabits running waters with varying light regimes in North America and Europe making this taxon a good candidate for study. The purpose of this research was to examine gene expression under light levels representing initial saturation, saturation and photoinhibition. Field collected gametophytes were acclimated to 265 μmol photon m-2 s-1 and then subjected to 75 μmol photon m-2 s-1 (low), 265 μmol photon m-2 s-1 (medium) and 600 μmol photon m-2 s-1 (high) for 72 hours. RNA was extracted in quadruplicate for each light level and sequenced on HiSeq2500 platform. All 12 replicates were used for de-novo assembly of the transcriptome. In the assembly process, only contigs identified as Rhodophyta were used, which represents a conservative approach to ensure that all possible contaminants such as epiphytes were removed for the differential gene expression analyses. Differential expression analysis between the Low vs Medium and High vs Medium, revealed the downregulation of chlorophyll synthesis pathway in Low light. The carbohydrate metabolism was downregulated in High light, while there was evidence of starch accumulation in Low light. Photoprotective mechanisms were evident in High light indicated by the upregulation of tocopherol and phylloquinone biosynthetic pathway. The transcription/translation process along with the protein translocation in endoplasmic reticulum were downregulated in High light while the genes involved in unfolded protein response and misfolded protein degradation were upregulated. Although it was expected that genes of the light harvesting cap and photosynthesis would be differentially expressed, little change was observed and may be due to physiological changes rather than changes at the molecular level in response to this short-term study. It was observed that chlorophyll synthesis, carbohydrate metabolism and photoprotective mechanisms were affected. 2020-09-28 English text Ohio University / OhioLINK http://rave.ohiolink.edu/etdc/view?acc_num=ohiou159671675380422 http://rave.ohiolink.edu/etdc/view?acc_num=ohiou159671675380422 unrestricted This thesis or dissertation is protected by copyright: all rights reserved. It may not be copied or redistributed beyond the terms of applicable copyright laws.
collection NDLTD
language English
sources NDLTD
topic Plant Biology
Photoinhibition
Photoprotection
Photoadaptation
spellingShingle Plant Biology
Photoinhibition
Photoprotection
Photoadaptation
Tiwari, Sunil
Effect of Different Light Intensities on Freshwater Red Algae Batrachospermum gelatinosum: A Transcriptomic Approach
author Tiwari, Sunil
author_facet Tiwari, Sunil
author_sort Tiwari, Sunil
title Effect of Different Light Intensities on Freshwater Red Algae Batrachospermum gelatinosum: A Transcriptomic Approach
title_short Effect of Different Light Intensities on Freshwater Red Algae Batrachospermum gelatinosum: A Transcriptomic Approach
title_full Effect of Different Light Intensities on Freshwater Red Algae Batrachospermum gelatinosum: A Transcriptomic Approach
title_fullStr Effect of Different Light Intensities on Freshwater Red Algae Batrachospermum gelatinosum: A Transcriptomic Approach
title_full_unstemmed Effect of Different Light Intensities on Freshwater Red Algae Batrachospermum gelatinosum: A Transcriptomic Approach
title_sort effect of different light intensities on freshwater red algae batrachospermum gelatinosum: a transcriptomic approach
publisher Ohio University / OhioLINK
publishDate 2020
url http://rave.ohiolink.edu/etdc/view?acc_num=ohiou159671675380422
work_keys_str_mv AT tiwarisunil effectofdifferentlightintensitiesonfreshwaterredalgaebatrachospermumgelatinosumatranscriptomicapproach
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