The evolution and expression of rbcL in holoparasitic sister genera, harveya hook. and hyobanche l. (orobanchaceae) and systematics and taxonomic revision of southern African species of harveya

Bibliographic Details
Main Author:	Randle, Christopher P.
Language:	English
Published:	The Ohio State University / OhioLINK 2004
Subjects:	Harveya Hyobanche parasitic plants rbcL ITS nrDNA systematics molecular evolution Orobanchaceae taxonomy phylogenetics Rubisco cpDNA
Online Access:	http://rave.ohiolink.edu/etdc/view?acc_num=osu1085582568

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spelling	ndltd-OhioLink-oai-etd.ohiolink.edu-osu10855825682021-08-03T05:48:42Z The evolution and expression of rbcL in holoparasitic sister genera, harveya hook. and hyobanche l. (orobanchaceae) and systematics and taxonomic revision of southern African species of harveya Randle, Christopher P. Harveya Hyobanche parasitic plants rbcL ITS nrDNA systematics molecular evolution Orobanchaceae taxonomy phylogenetics Rubisco cpDNA The evolution of holoparasitism in plants is often accompanied by reduction in structures and functions associated with photosynthesis and nutrient acquisition. This reduction in morphological and anatomical features occurs concomitantly with loss or truncation of photosynthesis-related genes freed from selective constraints. The chloroplast genome of many holoparasitic plants is drastically reduced in both the quantity and quality of genetic material. Harveya and Hyobanche (Orobanchaceae) are holoparasitic genera native to southern Africa. Hyobanche has undergone pseudogene formation at the chloroplast locus encoding the large subunit of the carbon-fixing holoenzyme Rubisco, rbcL, while Harveya maintains a seemingly functional copy of this gene, which is evolving under purifying selection. The regions flanking rbcL, the 5’- and 3’- Untranslated Regions, appear to be functional in both genera. However, Western blot analysis shows that the large subunit is present in both taxa, despite the presence of the pseudogene in Hyobanche. This may be explained by two hypotheses: 1) multiple copies of rbcL exist in Hyobanche, one of which codes for a functional protein, or 2) RNA transcripts are repaired by an as-yet unknown mechanism. Cloning and RNA experiments demonstrated that while there are indeed multiple copies of rbcL in Hyobanche, none of these codes for a functional protein. The hypothesis that Rubisco is obtained from host plants is discussed, as well as other possible functions of Rubisco in these putatively non-photosynthetic plants. Of 53 species names attributed to the genus Harveya, 35 represent species endemic to southern Africa. However, the genus has never been treated as a whole taxonomically, and the southern African species have not been revised since Hiern’s treatment of 1904. Species delimitations were reassessed using material collected in the field as well as specimens borrowed from herbaria, resulting in the reduction of the genus to twelve species native to southern Africa. A diagnostic key for identification of species was presented, and host-plant records, habitat, and geographical distributions were summarized for each species circumscribed. Molecular sequence data from the chloroplast and nucleus were used to test hypothesized relationships among these species, and the evolution of floral characteristics, host-plant ranges, and geographical origins. 2004-07-20 English text The Ohio State University / OhioLINK http://rave.ohiolink.edu/etdc/view?acc_num=osu1085582568 http://rave.ohiolink.edu/etdc/view?acc_num=osu1085582568 unrestricted This thesis or dissertation is protected by copyright: all rights reserved. It may not be copied or redistributed beyond the terms of applicable copyright laws.
collection	NDLTD
language	English
sources	NDLTD
topic	Harveya Hyobanche parasitic plants rbcL ITS nrDNA systematics molecular evolution Orobanchaceae taxonomy phylogenetics Rubisco cpDNA
spellingShingle	Harveya Hyobanche parasitic plants rbcL ITS nrDNA systematics molecular evolution Orobanchaceae taxonomy phylogenetics Rubisco cpDNA Randle, Christopher P. The evolution and expression of rbcL in holoparasitic sister genera, harveya hook. and hyobanche l. (orobanchaceae) and systematics and taxonomic revision of southern African species of harveya
author	Randle, Christopher P.
author_facet	Randle, Christopher P.
author_sort	Randle, Christopher P.
title	The evolution and expression of rbcL in holoparasitic sister genera, harveya hook. and hyobanche l. (orobanchaceae) and systematics and taxonomic revision of southern African species of harveya
title_short	The evolution and expression of rbcL in holoparasitic sister genera, harveya hook. and hyobanche l. (orobanchaceae) and systematics and taxonomic revision of southern African species of harveya
title_full	The evolution and expression of rbcL in holoparasitic sister genera, harveya hook. and hyobanche l. (orobanchaceae) and systematics and taxonomic revision of southern African species of harveya
title_fullStr	The evolution and expression of rbcL in holoparasitic sister genera, harveya hook. and hyobanche l. (orobanchaceae) and systematics and taxonomic revision of southern African species of harveya
title_full_unstemmed	The evolution and expression of rbcL in holoparasitic sister genera, harveya hook. and hyobanche l. (orobanchaceae) and systematics and taxonomic revision of southern African species of harveya
title_sort	evolution and expression of rbcl in holoparasitic sister genera, harveya hook. and hyobanche l. (orobanchaceae) and systematics and taxonomic revision of southern african species of harveya
publisher	The Ohio State University / OhioLINK
publishDate	2004
url	http://rave.ohiolink.edu/etdc/view?acc_num=osu1085582568
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_version_	1719425960316502016

The evolution and expression of rbcL in holoparasitic sister genera, harveya hook. and hyobanche l. (orobanchaceae) and systematics and taxonomic revision of southern African species of harveya

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