Functions and relationships of the TMM and SDD1 genes in arabidopsis stomatal development
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The Ohio State University / OhioLINK
2007
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ndltd-OhioLink-oai-etd.ohiolink.edu-osu11952297022021-08-03T05:52:50Z Functions and relationships of the TMM and SDD1 genes in arabidopsis stomatal development Bhave, Neela S. Arabidopsis Stomata development TOO MANY MOUTHS STOMATAL DENSITY AND DISTRIBUTION1 Asymmetric division Stomata are epidermal pores surrounded by two guard cells that regulate gas exchange between the shoot and the atmosphere. Stomata form and are spaced through asymmetric divisions controlled by TOO MANY MOUTHS (TMM) and STOMATAL DENSITY AND DISTRIBUTION1 (SDD1). The function of these genes have been characterized during leaf development where both restrict divisions and stomatal development. Unlike SDD1, TMM, a likely receptor, has different functions in different organs e.g. excess stomata form in tmm leaves but tmm stems lack stomata. In tmm stems, initial asymmetric divisions were normal, and the resulting precursor cells, meristemoids, appropriately expressed cell fate markers. However, instead of forming stomata, meristemoids arrested, dedifferentiated, and elongated. Thus asymmetric divisions are necessary but not sufficient for stem stomata and TMM is essential for the transition of a meristemoid into a guard mother cell. SDD1, a serine protease, also regulates asymmetric divisions. To study their genetic relationship, tmm-1 sdd1-2 double mutants were analyzed. Double mutant leaves had a complex phenotype, partly resembling each single mutant and partly exhibiting novel phenotypes. This suggests that these genes act either in separate genetic pathways or in ones that converge on division regulation. However, double mutant stems lack stomata and thus tmm is epistatic to sdd1-2. This suggests that TMM is downstream of SDD1 in the same genetic pathway in this organ. SDD1 overexpression reduces stomatal number and TMM is required for this phenotype, a result consistent with an epistatic relationship. TMM is also required in leaves for SDD1 overexpression phenotype suggesting that this gain-of-function phenotype results from SDD1 misexpression. In a wild type background the overexpression of TMM showed no abnormal phenotype. Finally, SDD1 regulates the size of the stomatal stem cell compartment marked by TMM expression. Thus nature of the interaction between SDD1 and TMM depends upon whether TMM acts as a positive regulator of precursor cell development or as a negative regulator of asymmetric divisions with the phenotype depending upon the organ. 2007-12-10 English text The Ohio State University / OhioLINK http://rave.ohiolink.edu/etdc/view?acc_num=osu1195229702 http://rave.ohiolink.edu/etdc/view?acc_num=osu1195229702 unrestricted This thesis or dissertation is protected by copyright: all rights reserved. It may not be copied or redistributed beyond the terms of applicable copyright laws. |
| collection |
NDLTD |
| language |
English |
| sources |
NDLTD |
| topic |
Arabidopsis Stomata development TOO MANY MOUTHS STOMATAL DENSITY AND DISTRIBUTION1 Asymmetric division |
| spellingShingle |
Arabidopsis Stomata development TOO MANY MOUTHS STOMATAL DENSITY AND DISTRIBUTION1 Asymmetric division Bhave, Neela S. Functions and relationships of the TMM and SDD1 genes in arabidopsis stomatal development |
| author |
Bhave, Neela S. |
| author_facet |
Bhave, Neela S. |
| author_sort |
Bhave, Neela S. |
| title |
Functions and relationships of the TMM and SDD1 genes in arabidopsis stomatal development |
| title_short |
Functions and relationships of the TMM and SDD1 genes in arabidopsis stomatal development |
| title_full |
Functions and relationships of the TMM and SDD1 genes in arabidopsis stomatal development |
| title_fullStr |
Functions and relationships of the TMM and SDD1 genes in arabidopsis stomatal development |
| title_full_unstemmed |
Functions and relationships of the TMM and SDD1 genes in arabidopsis stomatal development |
| title_sort |
functions and relationships of the tmm and sdd1 genes in arabidopsis stomatal development |
| publisher |
The Ohio State University / OhioLINK |
| publishDate |
2007 |
| url |
http://rave.ohiolink.edu/etdc/view?acc_num=osu1195229702 |
| work_keys_str_mv |
AT bhaveneelas functionsandrelationshipsofthetmmandsdd1genesinarabidopsisstomataldevelopment |
| _version_ |
1719427056786210816 |