Role of Genetics in Subgingival and Supragingival Bacterial Colonization
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The Ohio State University / OhioLINK
2009
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ndltd-OhioLink-oai-etd.ohiolink.edu-osu12434535462021-08-03T05:56:05Z Role of Genetics in Subgingival and Supragingival Bacterial Colonization Papapostolou, Anastasia Dental Care twins Bray-Curtis similarity index bacterial colonization periodontal health t-RFLP <p>The gingival sulcus and tooth consist of complex bacterial ecosystems, the majority of which are uncultivated. Although the sequence of oral bacterial colonization has been investigated, factors affecting this process, especially host genetics, are poorly understood. Twin studies provide a powerful model to study the contribution of genetics to diseases. Open-ended molecular methods are accurate and comprehensive approaches to compare complex microbial communities. The purpose of this study was to compare the subgingival and supragingival microbial profiles of periodontally healthy twins using a molecular method for bacterial profiling.</p><p>95 pairs of periodontally and systemically healthy twins, 18 to 36 years old, were selected. Zygosity information was elicited by questionnaire. Whole mouth subgingival and supragingival plaque samples were collected and pooled from each subject. Bacterial DNA was isolated from these samples and the 16S rRNA gene was amplified using polymerase chain reaction with fluorescent labeled broad-range eubacterial primers. The amplicons were restriction-digested using MspI and HhaI and Terminal Restriction Fragment Length Polymorphism Analysis (t-RFLP) was used to examine the composition of the microbial community in each individual. Similarity of microbial profiles between each twin pair was assessed using Bray-Curtis similarity index based on fragment peak areas. The number of shared species was also computed for each twin pair and compared between groups.</p><p>59% of the twin pairs were monozygotic, while 41% were dizygotic. Differences in race, smoking status and gender were not statistically significant between the twin groups. 29% of the subjects were current or former smokers. The supragingival community exhibited an average of 45% of similarity between all twin pairs and the subgingival biofilm 34% among all twin pairs; however, there was no significant difference between the two twin groups by Kruskal Wallis Analysis. No differences were observed after current and former smokers were excluded. Our findings also suggested that smoking or age were not contributing factors in the bacterial acquisition. The low number of fragments generated from the restriction digestion suggests that the diversity of a health-associated subgingival microbial community is low, with few dominant species. The microbial profiles of monozygotic twins were not significantly different from that of dizygotic twins, suggesting that the effects of host genetics on microbial colonization of supragingival plaque or subgingival biofilm are not evident in an established, health-compatible community. Further studies are required to elucidate the role of host genetics in early microbial colonization of the subgingival and supragingival plaque formation.</p> 2009-08-26 English text The Ohio State University / OhioLINK http://rave.ohiolink.edu/etdc/view?acc_num=osu1243453546 http://rave.ohiolink.edu/etdc/view?acc_num=osu1243453546 unrestricted This thesis or dissertation is protected by copyright: all rights reserved. It may not be copied or redistributed beyond the terms of applicable copyright laws. |
| collection |
NDLTD |
| language |
English |
| sources |
NDLTD |
| topic |
Dental Care twins Bray-Curtis similarity index bacterial colonization periodontal health t-RFLP |
| spellingShingle |
Dental Care twins Bray-Curtis similarity index bacterial colonization periodontal health t-RFLP Papapostolou, Anastasia Role of Genetics in Subgingival and Supragingival Bacterial Colonization |
| author |
Papapostolou, Anastasia |
| author_facet |
Papapostolou, Anastasia |
| author_sort |
Papapostolou, Anastasia |
| title |
Role of Genetics in Subgingival and Supragingival Bacterial Colonization |
| title_short |
Role of Genetics in Subgingival and Supragingival Bacterial Colonization |
| title_full |
Role of Genetics in Subgingival and Supragingival Bacterial Colonization |
| title_fullStr |
Role of Genetics in Subgingival and Supragingival Bacterial Colonization |
| title_full_unstemmed |
Role of Genetics in Subgingival and Supragingival Bacterial Colonization |
| title_sort |
role of genetics in subgingival and supragingival bacterial colonization |
| publisher |
The Ohio State University / OhioLINK |
| publishDate |
2009 |
| url |
http://rave.ohiolink.edu/etdc/view?acc_num=osu1243453546 |
| work_keys_str_mv |
AT papapostolouanastasia roleofgeneticsinsubgingivalandsupragingivalbacterialcolonization |
| _version_ |
1719428024931188736 |