Construction of a Nisin-Controlled Expression Vector, a Derivative of pMSP3535 for Alternative Selectable Marker
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2009
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| Online Access: | http://rave.ohiolink.edu/etdc/view?acc_num=osu1253160902 |
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ndltd-OhioLink-oai-etd.ohiolink.edu-osu12531609022021-08-03T05:57:06Z Construction of a Nisin-Controlled Expression Vector, a Derivative of pMSP3535 for Alternative Selectable Marker Rattanaprasert, Monchaya Microbiology Molecular Biology The plasmid pMPS3535 is a commonly-used nisin-inducible expression vector for homologous and heterologous genes in a variety of gram-positive bacteria. However, a number of limitations associate with this plasmid, including the use of the erythromycin resistance (Em<sup>r</sup>) marker. Em<sup>r</sup> is somewhat ubiquitous element in shuttle vectors, bacterial plasmids, and even in bacterial chromosomes, which limited its application in hosts naturally resistant to erythromycin and in combination with Em<sup>r</sup>-carrying vectors. To overcome this limitation, the Em<sup>r</sup> marker was displaced by an alternative tetracycline resistance maker encoded by <i>tetL</i> gene. The resulting vector, termed pMSP3535TLH, has been proved to be able to transform <i>Enterococcus faecalis</i> OG1RF, <i>Lactococcus lactis</i> 2301, and <i>Escherichia coli</i> DH5α to tetracycline resistance whose transformants were readily selected on selective plates supplemented with tetracycline. By taking advantage of the Em<sup>r</sup> marker removal to simultaneously excise a segment of <i>copF</i> gene, a negative regulator of copy number, the constructed vector has potential to become a high copy number derivative of the plasmid pMSP3535. 2009-09-30 English text The Ohio State University / OhioLINK http://rave.ohiolink.edu/etdc/view?acc_num=osu1253160902 http://rave.ohiolink.edu/etdc/view?acc_num=osu1253160902 unrestricted This thesis or dissertation is protected by copyright: all rights reserved. It may not be copied or redistributed beyond the terms of applicable copyright laws. |
| collection |
NDLTD |
| language |
English |
| sources |
NDLTD |
| topic |
Microbiology Molecular Biology |
| spellingShingle |
Microbiology Molecular Biology Rattanaprasert, Monchaya Construction of a Nisin-Controlled Expression Vector, a Derivative of pMSP3535 for Alternative Selectable Marker |
| author |
Rattanaprasert, Monchaya |
| author_facet |
Rattanaprasert, Monchaya |
| author_sort |
Rattanaprasert, Monchaya |
| title |
Construction of a Nisin-Controlled Expression Vector, a Derivative of pMSP3535 for Alternative Selectable Marker |
| title_short |
Construction of a Nisin-Controlled Expression Vector, a Derivative of pMSP3535 for Alternative Selectable Marker |
| title_full |
Construction of a Nisin-Controlled Expression Vector, a Derivative of pMSP3535 for Alternative Selectable Marker |
| title_fullStr |
Construction of a Nisin-Controlled Expression Vector, a Derivative of pMSP3535 for Alternative Selectable Marker |
| title_full_unstemmed |
Construction of a Nisin-Controlled Expression Vector, a Derivative of pMSP3535 for Alternative Selectable Marker |
| title_sort |
construction of a nisin-controlled expression vector, a derivative of pmsp3535 for alternative selectable marker |
| publisher |
The Ohio State University / OhioLINK |
| publishDate |
2009 |
| url |
http://rave.ohiolink.edu/etdc/view?acc_num=osu1253160902 |
| work_keys_str_mv |
AT rattanaprasertmonchaya constructionofanisincontrolledexpressionvectoraderivativeofpmsp3535foralternativeselectablemarker |
| _version_ |
1719428356387110912 |