Scaffolded DNA Origami Nanotechnology for Receptor Ligand Studies
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The Ohio State University / OhioLINK
2013
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| Online Access: | http://rave.ohiolink.edu/etdc/view?acc_num=osu1374169534 |
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ndltd-OhioLink-oai-etd.ohiolink.edu-osu13741695342021-08-03T06:18:40Z Scaffolded DNA Origami Nanotechnology for Receptor Ligand Studies Briggs, Emily N. Mechanical Engineering This research uses the recently developed nanotechnology scaffoldedDNA origami to build self-assembling nano-structures to study the mechanics and function of receptor-ligand interactions. Rigid DNA origami platforms were built with specific attachment points for ligands. The functionalization of the platform with specific sites for ligand attachment enabled control over number and arrangement of ligands at the molecular scale for controlled dosage of antigens to biological cells. Single cell imaging studies were performed to study the effects of co-stimulatory ligand number and density on signaling and proliferation of B cells. Other DNA origami structures have been fabricated to test the biophysical characteristics of receptor-ligand interactions. This device was designed to achieve high throughput single molecule force spectroscopy on a basic laboratory fluorescence microscope. These structures exploit the entropic elasticity of DNA to produce constant forces on receptor-ligand interactions for quantifying kinetic parameters. Proof-of-principle experiments were preformed with DNA-basedinteractio ns. 2013-10-02 English text The Ohio State University / OhioLINK http://rave.ohiolink.edu/etdc/view?acc_num=osu1374169534 http://rave.ohiolink.edu/etdc/view?acc_num=osu1374169534 unrestricted This thesis or dissertation is protected by copyright: some rights reserved. It is licensed for use under a Creative Commons license. Specific terms and permissions are available from this document's record in the OhioLINK ETD Center. |
| collection |
NDLTD |
| language |
English |
| sources |
NDLTD |
| topic |
Mechanical Engineering |
| spellingShingle |
Mechanical Engineering Briggs, Emily N. Scaffolded DNA Origami Nanotechnology for Receptor Ligand Studies |
| author |
Briggs, Emily N. |
| author_facet |
Briggs, Emily N. |
| author_sort |
Briggs, Emily N. |
| title |
Scaffolded DNA Origami Nanotechnology for Receptor Ligand Studies |
| title_short |
Scaffolded DNA Origami Nanotechnology for Receptor Ligand Studies |
| title_full |
Scaffolded DNA Origami Nanotechnology for Receptor Ligand Studies |
| title_fullStr |
Scaffolded DNA Origami Nanotechnology for Receptor Ligand Studies |
| title_full_unstemmed |
Scaffolded DNA Origami Nanotechnology for Receptor Ligand Studies |
| title_sort |
scaffolded dna origami nanotechnology for receptor ligand studies |
| publisher |
The Ohio State University / OhioLINK |
| publishDate |
2013 |
| url |
http://rave.ohiolink.edu/etdc/view?acc_num=osu1374169534 |
| work_keys_str_mv |
AT briggsemilyn scaffoldeddnaorigaminanotechnologyforreceptorligandstudies |
| _version_ |
1719434459871182848 |