Assembly and characterization of mesoscale DNA material systems based on periodic DNA origami arrays

Bibliographic Details
Main Author:	Turowski, Daniel J.
Language:	English
Published:	The Ohio State University / OhioLINK 2013
Subjects:	Biology Biomechanics Biomedical Engineering Mechanical Engineering DNA origami polymer mechanics persistence length
Online Access:	http://rave.ohiolink.edu/etdc/view?acc_num=osu1374169645

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spelling	ndltd-OhioLink-oai-etd.ohiolink.edu-osu13741696452021-08-03T06:18:40Z Assembly and characterization of mesoscale DNA material systems based on periodic DNA origami arrays Turowski, Daniel J. Biology Biomechanics Biomedical Engineering Mechanical Engineering DNA origami polymer mechanics persistence length DNA origami is a recently developed technology that manipulates DNA base-pairing to create nanoscale engineered structures on the order of 100 nm via molecular self-assembly. DNA origami nanostructures such as nanopores, molecular sensors, and protein templates have been applied to study different aspects of biomolecular and cellular systems. However, current applications of DNA origami are limited due to challenges in scaling nanoscale functionality up to relevant cellular and material scales. One method to create larger DNA origami structures is to assemble individual nanostructures into various periodic arrays, forming micron-scale 1D filaments or 2D materials. To better understand the assembly kinetics, Transmission Electron Microscopy (TEM) and fluorescence microscopy have been used to characterize 1D and 2D polymerization reactions in terms of growth rate and length distribution as functions of time, temperature and assembly reaction conditions. The mechanical properties of the resulting larger scale assemblies are also measured to better understand the mechanical properties of DNA origami structures. With an improved understanding of how to optimize the hierarchical assembly process, larger DNA origami structures can be created, opening the door for new materials applications. 2013-11-14 English text The Ohio State University / OhioLINK http://rave.ohiolink.edu/etdc/view?acc_num=osu1374169645 http://rave.ohiolink.edu/etdc/view?acc_num=osu1374169645 unrestricted This thesis or dissertation is protected by copyright: all rights reserved. It may not be copied or redistributed beyond the terms of applicable copyright laws.
collection	NDLTD
language	English
sources	NDLTD
topic	Biology Biomechanics Biomedical Engineering Mechanical Engineering DNA origami polymer mechanics persistence length
spellingShingle	Biology Biomechanics Biomedical Engineering Mechanical Engineering DNA origami polymer mechanics persistence length Turowski, Daniel J. Assembly and characterization of mesoscale DNA material systems based on periodic DNA origami arrays
author	Turowski, Daniel J.
author_facet	Turowski, Daniel J.
author_sort	Turowski, Daniel J.
title	Assembly and characterization of mesoscale DNA material systems based on periodic DNA origami arrays
title_short	Assembly and characterization of mesoscale DNA material systems based on periodic DNA origami arrays
title_full	Assembly and characterization of mesoscale DNA material systems based on periodic DNA origami arrays
title_fullStr	Assembly and characterization of mesoscale DNA material systems based on periodic DNA origami arrays
title_full_unstemmed	Assembly and characterization of mesoscale DNA material systems based on periodic DNA origami arrays
title_sort	assembly and characterization of mesoscale dna material systems based on periodic dna origami arrays
publisher	The Ohio State University / OhioLINK
publishDate	2013
url	http://rave.ohiolink.edu/etdc/view?acc_num=osu1374169645
work_keys_str_mv	AT turowskidanielj assemblyandcharacterizationofmesoscalednamaterialsystemsbasedonperiodicdnaorigamiarrays
_version_	1719434460238184448

Assembly and characterization of mesoscale DNA material systems based on periodic DNA origami arrays

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