Diverse Applications of Magnetotactic Bacteria
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| Language: | English |
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The Ohio State University / OhioLINK
2014
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| Online Access: | http://rave.ohiolink.edu/etdc/view?acc_num=osu1397571008 |
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ndltd-OhioLink-oai-etd.ohiolink.edu-osu1397571008 |
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oai_dc |
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NDLTD |
| language |
English |
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NDLTD |
| topic |
Education Environmental Science Genetics Microbiology Nanotechnology Science Education Secondary Education magnetotactic bacteria MTB nanochannel electroporation NEP nanofluidics magnetospirillum magneticum AMB-1 STEM education science education microbiology origin of life environmental isolation nanolithography genetic engineering magnetosensing |
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Education Environmental Science Genetics Microbiology Nanotechnology Science Education Secondary Education magnetotactic bacteria MTB nanochannel electroporation NEP nanofluidics magnetospirillum magneticum AMB-1 STEM education science education microbiology origin of life environmental isolation nanolithography genetic engineering magnetosensing Clark, Kylienne Annette Diverse Applications of Magnetotactic Bacteria |
| author |
Clark, Kylienne Annette |
| author_facet |
Clark, Kylienne Annette |
| author_sort |
Clark, Kylienne Annette |
| title |
Diverse Applications of Magnetotactic Bacteria |
| title_short |
Diverse Applications of Magnetotactic Bacteria |
| title_full |
Diverse Applications of Magnetotactic Bacteria |
| title_fullStr |
Diverse Applications of Magnetotactic Bacteria |
| title_full_unstemmed |
Diverse Applications of Magnetotactic Bacteria |
| title_sort |
diverse applications of magnetotactic bacteria |
| publisher |
The Ohio State University / OhioLINK |
| publishDate |
2014 |
| url |
http://rave.ohiolink.edu/etdc/view?acc_num=osu1397571008 |
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AT clarkkylienneannette diverseapplicationsofmagnetotacticbacteria |
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1719435997095133184 |
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ndltd-OhioLink-oai-etd.ohiolink.edu-osu13975710082021-08-03T06:23:49Z Diverse Applications of Magnetotactic Bacteria Clark, Kylienne Annette Education Environmental Science Genetics Microbiology Nanotechnology Science Education Secondary Education magnetotactic bacteria MTB nanochannel electroporation NEP nanofluidics magnetospirillum magneticum AMB-1 STEM education science education microbiology origin of life environmental isolation nanolithography genetic engineering magnetosensing Magnetotactic bacteria (MTB) compose a group of diverse, motile prokaryotes that have the ability to passively align themselves along Earth’s geomagnetic field due to the presence of intracellular organelles called magnetosomes. These unique organelles house nanometer-sized, membrane-enclosed, magnetic iron crystals of magnetite (Fe3O4), or less commonly, greigite (Fe3S4) that aid the bacteria in locating growth favorable conditions at or just below the oxic-anoxic transition zone of aquatic sediments. With implications in various fields, including evolutionary biology, biogeochemistry, and nanotechnology, research on MTB and their magnetosomes has steadily increased since they were described by Richard Blakemore in 1975. The extraordinary diversity and unique features of MTB directly translate to educational topics that can be applied to Science, Technology, Mathematics, and Engineering (STEM) requirements within the classroom. The development of a distributable introductory microbiology kit, focused on magnetotactic bacteria, would make the study of MTB and related STEM subjects attainable for secondary school and even primary school aged students. Such a kit would include all the required sterile materials and specific, illustrated instructions for the culturing of magnetotactic species Magnetospirillum magneticum AMB-1, the isolation of MTB from the environment, and observing MTB samples with light microscopy. Magnetotactic bacteria have advantage over other microorganism in that they are non-pathogenic, motile, relatively easy to isolate from the environment, highly interactive, and yield seamless transitions to countless other STEM dimensions. These qualities are important in providing the flexibility essential for teachers who are required to cover a variety of core curriculum topics, as well as for engaging the wondering minds of students within this age group. It is the objective of such a kit to provide a positive experience with microbiology that will eventually inspire students to pursue an advanced degree within this vital field and other STEM fields related to it. There are several research endeavors that require the ability to insert foreign DNA into magnetotactic bacteria. While a number of techniques have been developed to transform prokaryotic cells (i.e., targeted conjugation, bulk electroporation, heat shock methods, etc.) limited success has resulted when they are applied to MTB. An emerging method, nanochannel electroporation (NEP), has successfully been tested on mammalian, yeast, and bacterial cells but has not, until now, been used to transform MTB. NEP minimizes cell trauma by reducing the cell membrane area needing to be affected and controlling the dose of potentially toxic agents delivered to individual cells; both of which are cited challenges within traditional transformation methods.This research has demonstrated the capability and effectiveness of NEP to inject foreign molecules (PI dye) into magnetotactic bacteria. The next step is to use this technique to insert foreign DNA (i.e., a plasmid) into MTB. Initial studies conducted by this laboratory using M. magneticum AMB-1 and a plasmid (pAmCyan) have thus far been inconclusive. More studies need to be conducted in order to produce a competent culture of AMB-1 that can take up the pAmCyan plasmid. Doing so will expand the application of both NEP and MTB in nanotechnologies. 2014-09-02 English text The Ohio State University / OhioLINK http://rave.ohiolink.edu/etdc/view?acc_num=osu1397571008 http://rave.ohiolink.edu/etdc/view?acc_num=osu1397571008 unrestricted This thesis or dissertation is protected by copyright: all rights reserved. It may not be copied or redistributed beyond the terms of applicable copyright laws. |