The cloning and cellular basis of a novel tomato fruit weight gene: Cell Size Regulator (FW11.3/CSR)
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The Ohio State University / OhioLINK
2015
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| Online Access: | http://rave.ohiolink.edu/etdc/view?acc_num=osu1437584743 |
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ndltd-OhioLink-oai-etd.ohiolink.edu-osu14375847432021-08-03T06:32:20Z The cloning and cellular basis of a novel tomato fruit weight gene: Cell Size Regulator (FW11.3/CSR) Mu, Qi Horticulture Agriculture Plant Biology QTL fruit size cell size endoreduplication tomato Domestication and selective breeding of the tomato (Solanum lycopersicum) has produced an extremely large amount of diversity in fruit size and shape. Tomato fruit size is an important agronomic trait for commercial production as well as consumer preference. Fruit size, measured by weight, is determined by cell number and cell size, resulting from cell division and cell expansion, respectively. Of the 29 loci controlling tomato fruit weight, 2 have been cloned: FW2.2/CNR and FW3.2/SlKLUH. These loci were identified as contributing to the regulation of cell number via two different mechanisms, which ultimately led to fruit size alteration. A novel fruit weight locus fruit weight 11.3 (fw11.3) was cloned and the likely cellular basis for fruit enlargement was identified in this study. The fw11.3 locus was fine-mapped to a 13kb region on chromosome 11. DNA polymorphism analysis, association analysis and transgenic complementation tests demonstrated that Solyc11g71940 underlies fw11.3. The deletion leading to a 194 amino-acid truncation at the C-terminus was the most likely cause of fruit enlargement. Gene expression analysis revealed a dynamic regulation of Solyc11g71940 especially in the later stage of fruit development. Cell size and ploidy changes were observed in the fruit pericarp tissue of fw11.3 near isogenic lines (NILs), indicating a novel function of cell size regulation leading to fruit enlargement. Thus, we propose to rename FW11.3, cell size regulator (CSR). Importantly, the ploidy analysis provided in this study linked endoreduplication to an increase in cell size and ultimately to fruit enlargement, this is pivotal to understanding the mechanism for fruit enlargement during crop domestication. Phylogenic analysis revealed a unique propagation of CSR in the Solanaceae family with three additional paralogs. Few pleiotropic phenotypic differences were observed, except for blossom-end rot, where a significantly higher incidence rate was found to be associated with CSR. 2015-10-15 English text The Ohio State University / OhioLINK http://rave.ohiolink.edu/etdc/view?acc_num=osu1437584743 http://rave.ohiolink.edu/etdc/view?acc_num=osu1437584743 unrestricted This thesis or dissertation is protected by copyright: some rights reserved. It is licensed for use under a Creative Commons license. Specific terms and permissions are available from this document's record in the OhioLINK ETD Center. |
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NDLTD |
| language |
English |
| sources |
NDLTD |
| topic |
Horticulture Agriculture Plant Biology QTL fruit size cell size endoreduplication tomato |
| spellingShingle |
Horticulture Agriculture Plant Biology QTL fruit size cell size endoreduplication tomato Mu, Qi The cloning and cellular basis of a novel tomato fruit weight gene: Cell Size Regulator (FW11.3/CSR) |
| author |
Mu, Qi |
| author_facet |
Mu, Qi |
| author_sort |
Mu, Qi |
| title |
The cloning and cellular basis of a novel tomato fruit weight gene: Cell Size Regulator (FW11.3/CSR) |
| title_short |
The cloning and cellular basis of a novel tomato fruit weight gene: Cell Size Regulator (FW11.3/CSR) |
| title_full |
The cloning and cellular basis of a novel tomato fruit weight gene: Cell Size Regulator (FW11.3/CSR) |
| title_fullStr |
The cloning and cellular basis of a novel tomato fruit weight gene: Cell Size Regulator (FW11.3/CSR) |
| title_full_unstemmed |
The cloning and cellular basis of a novel tomato fruit weight gene: Cell Size Regulator (FW11.3/CSR) |
| title_sort |
cloning and cellular basis of a novel tomato fruit weight gene: cell size regulator (fw11.3/csr) |
| publisher |
The Ohio State University / OhioLINK |
| publishDate |
2015 |
| url |
http://rave.ohiolink.edu/etdc/view?acc_num=osu1437584743 |
| work_keys_str_mv |
AT muqi thecloningandcellularbasisofanoveltomatofruitweightgenecellsizeregulatorfw113csr AT muqi cloningandcellularbasisofanoveltomatofruitweightgenecellsizeregulatorfw113csr |
| _version_ |
1719438714629783552 |