Effects of Akkermansia muciniphila Supplementation on Markers of Intestinal Permeability in Dogs Following Antibiotic Treatment
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The Ohio State University / OhioLINK
2017
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| Online Access: | http://rave.ohiolink.edu/etdc/view?acc_num=osu1488318772663017 |
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ndltd-OhioLink-oai-etd.ohiolink.edu-osu14883187726630172021-08-03T07:00:57Z Effects of Akkermansia muciniphila Supplementation on Markers of Intestinal Permeability in Dogs Following Antibiotic Treatment Jugan, Maria Christine Veterinary Services Akkermansia muciniphila gastrointestinal permeability antibiotics canine fecal score Diarrhea and other sequelae of gastrointestinal hyperpermeability are common complications of antibiotic therapy. Akkermansia muciniphila is a mucin-degrading bacterium, positively associated with gastrointestinal epithelial health and decreased permeability. The objectives of this study were to measure effects of oral Akkermansia administration on markers of gastrointestinal permeability following antibiotic administration.Eight healthy, purpose-bred dogs were randomized to receive either Akkermansia (109 CFU/kg; N=4) or vehicle (N=4) for 6 days following a 7-day course of metronidazole. After a 20-day washout, dogs were crossed-over to the alternate treatment. After an additional 20-day washout, the experiment was repeated with amoxicillin-clavulanate. Fecal Akkermansia qPCR and plasma concentrations (measured by ELISA) of cytokeratin-18, lipopolysaccharide, and glucagon-like peptides (GLP-1, GLP-2) were measured at baseline (T0), post-antibiotic (T1), and post-treatment (vehicle or Akkermansia; T2). For each antibiotic, absolute or delta concentrations were compared between time-points using paired samples t test.Akkermansia was detected in feces in 7/8 dogs following supplementation (T2) but not at T0 or T1. Delta (T2-T1) cytokeratin-18 after metronidazole was significantly lower on vehicle (-0.27 ng/ml) versus Akkermansia (2.4 ng/ml; p=0.03). Cytokeratin-18 concentrations tended to decrease from T0 to T1 on amoxicillin-clavulanate (p=0.05). Post-prandial GLP-1 concentrations (38.2 pM) were higher than pre-prandial (15.5 pM) concentrations. No adverse side-effects or other significant biomarker alterations were noted. Akkermansia muciniphila PCR detection suggested successful gastrointestinal transit following oral supplementation in dogs, with an effect on gastrointestinal epithelium based on plasma cytokeratin-18 alterations. Further study is needed to determine impact in dogs with naturally-occurring disease. 2017-05-26 English text The Ohio State University / OhioLINK http://rave.ohiolink.edu/etdc/view?acc_num=osu1488318772663017 http://rave.ohiolink.edu/etdc/view?acc_num=osu1488318772663017 unrestricted This thesis or dissertation is protected by copyright: all rights reserved. It may not be copied or redistributed beyond the terms of applicable copyright laws. |
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NDLTD |
| language |
English |
| sources |
NDLTD |
| topic |
Veterinary Services Akkermansia muciniphila gastrointestinal permeability antibiotics canine fecal score |
| spellingShingle |
Veterinary Services Akkermansia muciniphila gastrointestinal permeability antibiotics canine fecal score Jugan, Maria Christine Effects of Akkermansia muciniphila Supplementation on Markers of Intestinal Permeability in Dogs Following Antibiotic Treatment |
| author |
Jugan, Maria Christine |
| author_facet |
Jugan, Maria Christine |
| author_sort |
Jugan, Maria Christine |
| title |
Effects of Akkermansia muciniphila Supplementation on Markers of Intestinal Permeability in Dogs Following Antibiotic Treatment |
| title_short |
Effects of Akkermansia muciniphila Supplementation on Markers of Intestinal Permeability in Dogs Following Antibiotic Treatment |
| title_full |
Effects of Akkermansia muciniphila Supplementation on Markers of Intestinal Permeability in Dogs Following Antibiotic Treatment |
| title_fullStr |
Effects of Akkermansia muciniphila Supplementation on Markers of Intestinal Permeability in Dogs Following Antibiotic Treatment |
| title_full_unstemmed |
Effects of Akkermansia muciniphila Supplementation on Markers of Intestinal Permeability in Dogs Following Antibiotic Treatment |
| title_sort |
effects of akkermansia muciniphila supplementation on markers of intestinal permeability in dogs following antibiotic treatment |
| publisher |
The Ohio State University / OhioLINK |
| publishDate |
2017 |
| url |
http://rave.ohiolink.edu/etdc/view?acc_num=osu1488318772663017 |
| work_keys_str_mv |
AT juganmariachristine effectsofakkermansiamuciniphilasupplementationonmarkersofintestinalpermeabilityindogsfollowingantibiotictreatment |
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