Green tea extract protects against diethylnitrosamine-mediated liver injury and cell proliferation by attenuating STAT3 and iNOS expression in high fat-induced obese mice with nonalcoholic steatohepatitis

Green tea extract protects against diethylnitrosamine-mediated liver injury and cell proliferation by attenuating STAT3 and iNOS expression in high fat-induced obese mice with nonalcoholic steatohepatitis

Bibliographic Details
Main Author: Kim, Joshua B.
Language:English
Published: The Ohio State University / OhioLINK 2017
Subjects:
Nutrition
diethylnitrosamine
green tea
hepatocellular carcinoma
nonalcoholic steatohepatitis
obesity
Online Access:http://rave.ohiolink.edu/etdc/view?acc_num=osu1502898913421724
id ndltd-OhioLink-oai-etd.ohiolink.edu-osu1502898913421724
record_format oai_dc
spelling ndltd-OhioLink-oai-etd.ohiolink.edu-osu15028989134217242021-08-03T07:03:46Z Green tea extract protects against diethylnitrosamine-mediated liver injury and cell proliferation by attenuating STAT3 and iNOS expression in high fat-induced obese mice with nonalcoholic steatohepatitis Kim, Joshua B. Nutrition diethylnitrosamine green tea hepatocellular carcinoma nonalcoholic steatohepatitis obesity Nonalcoholic steatohepatitis (NASH) increases hepatocellular carcinoma (HCC) risk by increasing inflammation and oncogenesis. Antiinflammatory activities of green tea extract (GTE) protect against dietary high-fat (HF)-induced NASH. I hypothesized that antiinflammatory and anti-oncogenic activities of GTE during NASH would also prevent diethylnitrosamine (DEN)-induced development towards HCC. Male C57BL/6J mice (4 wk old) were fed a HF diet devoid of, or supplemented with, GTE at 2% (w/w) and received once weekly intraperitoneal injections of saline vehicle or DEN (60 mg/kg; 5 and 7 wk old) until 25 wk old. Gross pathological observation indicated no tumors, as expected. GTE protected against obesity-associated parameters, histological and biochemical evidence of NASH, and hepatic TNFa and MCP-1 expression in both saline- and DEN-injected mice (P<0.05). GTE attenuated serum alanine aminotransferase (ALT) activity, hepatic malondialdehyde (MDA), hepatic iNOS and survivin mRNA expression, signal transducer and activator of transcription 3 (STAT3), and hepatocyte proliferating cell nuclear antigen (PCNA) otherwise exacerbated by DEN. Hepatic GSTP protein expression increased in mice fed GTE. Serum ALT was correlated (r = 0.71-0.83; P<0.0001) with MDA, iNOS, STAT3, survivin and PCNA. iNOS correlated with PCNA, STAT3 (r = 0.64-0.65; P<0.05) and survivin (r = 0.66; P<0.0001), suggesting that iNOS-induced inflammation regulates liver injury and oncogenic cell proliferation. GTE lowers NASH- and DEN-mediated HCC risk by increasing GSTP and attenuating iNOS-mediated liver injury and survivin-mediated cell proliferation. 2017 English text The Ohio State University / OhioLINK http://rave.ohiolink.edu/etdc/view?acc_num=osu1502898913421724 http://rave.ohiolink.edu/etdc/view?acc_num=osu1502898913421724 unrestricted This thesis or dissertation is protected by copyright: all rights reserved. It may not be copied or redistributed beyond the terms of applicable copyright laws.
collection NDLTD
language English
sources NDLTD
topic Nutrition
diethylnitrosamine
green tea
hepatocellular carcinoma
nonalcoholic steatohepatitis
obesity
spellingShingle Nutrition
diethylnitrosamine
green tea
hepatocellular carcinoma
nonalcoholic steatohepatitis
obesity
Kim, Joshua B.
Green tea extract protects against diethylnitrosamine-mediated liver injury and cell proliferation by attenuating STAT3 and iNOS expression in high fat-induced obese mice with nonalcoholic steatohepatitis
author Kim, Joshua B.
author_facet Kim, Joshua B.
author_sort Kim, Joshua B.
title Green tea extract protects against diethylnitrosamine-mediated liver injury and cell proliferation by attenuating STAT3 and iNOS expression in high fat-induced obese mice with nonalcoholic steatohepatitis
title_short Green tea extract protects against diethylnitrosamine-mediated liver injury and cell proliferation by attenuating STAT3 and iNOS expression in high fat-induced obese mice with nonalcoholic steatohepatitis
title_full Green tea extract protects against diethylnitrosamine-mediated liver injury and cell proliferation by attenuating STAT3 and iNOS expression in high fat-induced obese mice with nonalcoholic steatohepatitis
title_fullStr Green tea extract protects against diethylnitrosamine-mediated liver injury and cell proliferation by attenuating STAT3 and iNOS expression in high fat-induced obese mice with nonalcoholic steatohepatitis
title_full_unstemmed Green tea extract protects against diethylnitrosamine-mediated liver injury and cell proliferation by attenuating STAT3 and iNOS expression in high fat-induced obese mice with nonalcoholic steatohepatitis
title_sort green tea extract protects against diethylnitrosamine-mediated liver injury and cell proliferation by attenuating stat3 and inos expression in high fat-induced obese mice with nonalcoholic steatohepatitis
publisher The Ohio State University / OhioLINK
publishDate 2017
url http://rave.ohiolink.edu/etdc/view?acc_num=osu1502898913421724
work_keys_str_mv AT kimjoshuab greenteaextractprotectsagainstdiethylnitrosaminemediatedliverinjuryandcellproliferationbyattenuatingstat3andinosexpressioninhighfatinducedobesemicewithnonalcoholicsteatohepatitis
_version_ 1719452837119787008

Similar Items