Development of Solid-State NMR Methodologies for Protein Structure Determination based on Paramagnetic Tagging
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The Ohio State University / OhioLINK
2018
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| Online Access: | http://rave.ohiolink.edu/etdc/view?acc_num=osu1534438273233685 |
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ndltd-OhioLink-oai-etd.ohiolink.edu-osu15344382732336852021-08-03T07:08:18Z Development of Solid-State NMR Methodologies for Protein Structure Determination based on Paramagnetic Tagging Mukhopadhyay, Dwaipayan Chemistry solid-state NMR paramagnetic NMR protein structure In recent years solid-state NMR has emerged as a valuable tool for elucidating structure and dynamics of large biomolecular systems, especially suited for application in systems ranging from amyloid fibrils, membrane proteins to large protein ligand complexes inaccessible by more traditional structure determination techniques e.g. X-ray crystallography and solution-state NMR. Essential in determination of a biomolecular structure is the acquisition of numerous high quality interatomic distance restraints. However, conventional dipolar coupling based methods lead to significant challenges for accurate determination of critical long range distances. An alternative method to overcome this limitation is the incorporation of paramagnetic centers in proteins and utilization of the very large electron-nucleus couplings. In this dissertation, we combine state of the art proton-detection techniques with development of novel rigid transition metal binding tags, to improve the quality of long range distance restraints generated by measurement of site-specific paramagnetic relaxation enhancements in solid-state NMR. 2018 English text The Ohio State University / OhioLINK http://rave.ohiolink.edu/etdc/view?acc_num=osu1534438273233685 http://rave.ohiolink.edu/etdc/view?acc_num=osu1534438273233685 restricted--full text unavailable until 2023-12-17 This thesis or dissertation is protected by copyright: all rights reserved. It may not be copied or redistributed beyond the terms of applicable copyright laws. |
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NDLTD |
| language |
English |
| sources |
NDLTD |
| topic |
Chemistry solid-state NMR paramagnetic NMR protein structure |
| spellingShingle |
Chemistry solid-state NMR paramagnetic NMR protein structure Mukhopadhyay, Dwaipayan Development of Solid-State NMR Methodologies for Protein Structure Determination based on Paramagnetic Tagging |
| author |
Mukhopadhyay, Dwaipayan |
| author_facet |
Mukhopadhyay, Dwaipayan |
| author_sort |
Mukhopadhyay, Dwaipayan |
| title |
Development of Solid-State NMR Methodologies for Protein Structure Determination based on Paramagnetic Tagging |
| title_short |
Development of Solid-State NMR Methodologies for Protein Structure Determination based on Paramagnetic Tagging |
| title_full |
Development of Solid-State NMR Methodologies for Protein Structure Determination based on Paramagnetic Tagging |
| title_fullStr |
Development of Solid-State NMR Methodologies for Protein Structure Determination based on Paramagnetic Tagging |
| title_full_unstemmed |
Development of Solid-State NMR Methodologies for Protein Structure Determination based on Paramagnetic Tagging |
| title_sort |
development of solid-state nmr methodologies for protein structure determination based on paramagnetic tagging |
| publisher |
The Ohio State University / OhioLINK |
| publishDate |
2018 |
| url |
http://rave.ohiolink.edu/etdc/view?acc_num=osu1534438273233685 |
| work_keys_str_mv |
AT mukhopadhyaydwaipayan developmentofsolidstatenmrmethodologiesforproteinstructuredeterminationbasedonparamagnetictagging |
| _version_ |
1719454525668982784 |