Investigating the Heterogeneities of Clathrin Dynamics

Bibliographic Details
Main Author:	Willy, Nathan
Language:	English
Published:	The Ohio State University / OhioLINK 2019
Subjects:	Cellular Biology Biophysics clathrin mediated endocytosis membrane tension clathrin force spectroscopy
Online Access:	http://rave.ohiolink.edu/etdc/view?acc_num=osu1546466828242355

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spelling	ndltd-OhioLink-oai-etd.ohiolink.edu-osu15464668282423552021-08-03T07:09:26Z Investigating the Heterogeneities of Clathrin Dynamics Willy, Nathan Cellular Biology Biophysics clathrin mediated endocytosis membrane tension clathrin force spectroscopy During Clathrin Mediated Endocytosis (CME), Clathrin triskelia join to form apolyhedral cage on the interior of a cell’s plasma membrane. As this cage is built,curvature of the membrane increases until a small piece is pinched off andinternalized. This process serves a variety of important functions and is common andessential to all eukaryotic life. As such, it has been the most studied endocytic pathwaysince its discovery in the mid-1970s. Even so, fundamental questions remain about themechanics of CME, which can vary wildly. This work seeks to explore theheterogeneities of clathrin coat structure and dynamics. The exact nature of thecurvature-inducing forces has yet to be elucidated. But, no matter how the forces aregenerated, they will be directly opposed by any in-line tension held within the cellmembrane. Our work has shown that CME dynamics are detectably altered by bothspatial and temporal variations in membrane tension. Thus, endocytosis rates arestrongly regulated by forces exerted on the membrane. As a result, observation ofclathrin or clathrin coat components is sufficient to make meaningful commentary onchanges in membrane tension. We are excited to propose analysis of clathrin dynamicsas a convenient, widely accessible, and non-invasive method of probing membranetension. Interest in membrane tension and mechano-sensation in general has beengrowing as more and more evidence has shown its importance to determining cellbehavior both in isolation and in vivo. In our effort to better understand the variations,we also begin to explore the often neglected, non-canonical clathrin coat structures,developing a novel imaging technique and giving more insight into how clathrinfunctions among different cell types. 2019-07-11 English text The Ohio State University / OhioLINK http://rave.ohiolink.edu/etdc/view?acc_num=osu1546466828242355 http://rave.ohiolink.edu/etdc/view?acc_num=osu1546466828242355 unrestricted This thesis or dissertation is protected by copyright: all rights reserved. It may not be copied or redistributed beyond the terms of applicable copyright laws.
collection	NDLTD
language	English
sources	NDLTD
topic	Cellular Biology Biophysics clathrin mediated endocytosis membrane tension clathrin force spectroscopy
spellingShingle	Cellular Biology Biophysics clathrin mediated endocytosis membrane tension clathrin force spectroscopy Willy, Nathan Investigating the Heterogeneities of Clathrin Dynamics
author	Willy, Nathan
author_facet	Willy, Nathan
author_sort	Willy, Nathan
title	Investigating the Heterogeneities of Clathrin Dynamics
title_short	Investigating the Heterogeneities of Clathrin Dynamics
title_full	Investigating the Heterogeneities of Clathrin Dynamics
title_fullStr	Investigating the Heterogeneities of Clathrin Dynamics
title_full_unstemmed	Investigating the Heterogeneities of Clathrin Dynamics
title_sort	investigating the heterogeneities of clathrin dynamics
publisher	The Ohio State University / OhioLINK
publishDate	2019
url	http://rave.ohiolink.edu/etdc/view?acc_num=osu1546466828242355
work_keys_str_mv	AT willynathan investigatingtheheterogeneitiesofclathrindynamics
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Investigating the Heterogeneities of Clathrin Dynamics

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