High Force Applications of DNA Origami Devices
| Main Author: | |
|---|---|
| Language: | English |
| Published: |
The Ohio State University / OhioLINK
2021
|
| Subjects: | |
| Online Access: | http://rave.ohiolink.edu/etdc/view?acc_num=osu1619092851712077 |
| id |
ndltd-OhioLink-oai-etd.ohiolink.edu-osu1619092851712077 |
|---|---|
| record_format |
oai_dc |
| spelling |
ndltd-OhioLink-oai-etd.ohiolink.edu-osu16190928517120772021-10-16T05:25:16Z High Force Applications of DNA Origami Devices Darcy, Michael Augusto Biophysics Biochemistry Physics Nanoscience DNA nanotechnology force spectroscopy biophysics chromatin Hidden Markov Modeling fluorescence microscopy TR-FRET biochemistry nanocaliper DNA origami high force application direct force measurement transition kinetics The ability to apply and measure high forces (≥10pN) on the nanometer scale is critical to theongoing development of nanomedicine, molecular robotics, and the understanding of biologicalprocesses such as chromatin condensation, membrane deformation, and molecular motors [1] [2][3]. Current force spectroscopy techniques rely on micron-sized handles to apply forces, whichcan limit applications within nanofluidic devices or cellular environments [4]. To overcome theselimitations, I used deoxyribonucleic (DNA) origami to self-assemble a nanocaliper, building onprevious designs[5] [6]. I characterize the nanocaliper via a short double-stranded (ds)DNA witheach strand attached to opposite arms of the device, via device equilibrium state, output force,and dynamics, to understand the effects of sequence, vertex design, and strut length on the deviceproperties. I also produce nucleosomes, hexasomes, and an alternate dsDNA, which were thenmeasured in the device, yielding mechanistic insight into the free energy landscape of each. Imeasure forces greater than 20 pN applied by the device with a nanometer dynamic range and 1 to 10 pN/nm stiffness. These high performing characteristics which expand the capabilities of existing force spectroscopy techniques as well as those of DNA origami devices. 2021-10-05 English text The Ohio State University / OhioLINK http://rave.ohiolink.edu/etdc/view?acc_num=osu1619092851712077 http://rave.ohiolink.edu/etdc/view?acc_num=osu1619092851712077 unrestricted This thesis or dissertation is protected by copyright: all rights reserved. It may not be copied or redistributed beyond the terms of applicable copyright laws. |
| collection |
NDLTD |
| language |
English |
| sources |
NDLTD |
| topic |
Biophysics Biochemistry Physics Nanoscience DNA nanotechnology force spectroscopy biophysics chromatin Hidden Markov Modeling fluorescence microscopy TR-FRET biochemistry nanocaliper DNA origami high force application direct force measurement transition kinetics |
| spellingShingle |
Biophysics Biochemistry Physics Nanoscience DNA nanotechnology force spectroscopy biophysics chromatin Hidden Markov Modeling fluorescence microscopy TR-FRET biochemistry nanocaliper DNA origami high force application direct force measurement transition kinetics Darcy, Michael Augusto High Force Applications of DNA Origami Devices |
| author |
Darcy, Michael Augusto |
| author_facet |
Darcy, Michael Augusto |
| author_sort |
Darcy, Michael Augusto |
| title |
High Force Applications of DNA Origami Devices |
| title_short |
High Force Applications of DNA Origami Devices |
| title_full |
High Force Applications of DNA Origami Devices |
| title_fullStr |
High Force Applications of DNA Origami Devices |
| title_full_unstemmed |
High Force Applications of DNA Origami Devices |
| title_sort |
high force applications of dna origami devices |
| publisher |
The Ohio State University / OhioLINK |
| publishDate |
2021 |
| url |
http://rave.ohiolink.edu/etdc/view?acc_num=osu1619092851712077 |
| work_keys_str_mv |
AT darcymichaelaugusto highforceapplicationsofdnaorigamidevices |
| _version_ |
1719490050778988544 |