A chemical indirect quantification method for 5-hydroxymethylcytosine

Bibliographic Details
Main Author:	Premnauth, Gurdat
Language:	English
Published:	University of Toledo / OhioLINK 2016
Subjects:	Chemistry 5-hydroxymethylcytosine epigenetics oxidative labeling DNA modification quantification
Online Access:	http://rave.ohiolink.edu/etdc/view?acc_num=toledo1470434200

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spelling	ndltd-OhioLink-oai-etd.ohiolink.edu-toledo14704342002021-08-03T06:38:16Z A chemical indirect quantification method for 5-hydroxymethylcytosine Premnauth, Gurdat Chemistry 5-hydroxymethylcytosine epigenetics oxidative labeling DNA modification quantification 5-Hydroxymethylcytosine (5-hmC) results from the oxidation of 5-methylcytosine (5-mC) by one of the ten-eleven translocation enzymes. This moiety is considered a major epigenetic modification. Although research on DNA methylation is well documented, there is still a lot to learn about DNA hydroxymethylation. Multiples studies have shown the variation of 5-hmC in multiple diseases including a wide range of cancers and neurodegenerative and neurodevelopmental diseases. This fact supports the hypothesis that 5-hmC plays an important role in these diseases, thus making its occurrence a major research target. Although its biological role is still under investigation, multiple quantification methods have been developed to provide a better understanding of its role in different disease states.The method currently under development in our laboratory involves the oxidative labeling of 5-hmC. This is done through the selective oxidation of 5-hmC to 5-formylcytosine (5-foC), using efficient and mild oxidants followed by the formation of a stable conjugate with a biotin aminooxy linker, affinity enrichment, and detection. 2016 English text University of Toledo / OhioLINK http://rave.ohiolink.edu/etdc/view?acc_num=toledo1470434200 http://rave.ohiolink.edu/etdc/view?acc_num=toledo1470434200 unrestricted This thesis or dissertation is protected by copyright: all rights reserved. It may not be copied or redistributed beyond the terms of applicable copyright laws.
collection	NDLTD
language	English
sources	NDLTD
topic	Chemistry 5-hydroxymethylcytosine epigenetics oxidative labeling DNA modification quantification
spellingShingle	Chemistry 5-hydroxymethylcytosine epigenetics oxidative labeling DNA modification quantification Premnauth, Gurdat A chemical indirect quantification method for 5-hydroxymethylcytosine
author	Premnauth, Gurdat
author_facet	Premnauth, Gurdat
author_sort	Premnauth, Gurdat
title	A chemical indirect quantification method for 5-hydroxymethylcytosine
title_short	A chemical indirect quantification method for 5-hydroxymethylcytosine
title_full	A chemical indirect quantification method for 5-hydroxymethylcytosine
title_fullStr	A chemical indirect quantification method for 5-hydroxymethylcytosine
title_full_unstemmed	A chemical indirect quantification method for 5-hydroxymethylcytosine
title_sort	chemical indirect quantification method for 5-hydroxymethylcytosine
publisher	University of Toledo / OhioLINK
publishDate	2016
url	http://rave.ohiolink.edu/etdc/view?acc_num=toledo1470434200
work_keys_str_mv	AT premnauthgurdat achemicalindirectquantificationmethodfor5hydroxymethylcytosine AT premnauthgurdat chemicalindirectquantificationmethodfor5hydroxymethylcytosine
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A chemical indirect quantification method for 5-hydroxymethylcytosine

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