Studying the RNA-Recognition Site of RNase U2 for a More Diverse Bioanalytical Toolbox in RNA Modification Mapping
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University of Cincinnati / OhioLINK
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Online Access: | http://rave.ohiolink.edu/etdc/view?acc_num=ucin156387231304478 |
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ndltd-OhioLink-oai-etd.ohiolink.edu-ucin1563872313044782021-08-16T05:10:40Z Studying the RNA-Recognition Site of RNase U2 for a More Diverse Bioanalytical Toolbox in RNA Modification Mapping Solivio, Beulah Mae Ann Analytical Chemistry RNA Modification Mapping Ribonuclease Mass spectrometry RNase U2 Enzyme This dissertation is focused on enhancing the available tools for RNA analysis in terms of ribonucleases (RNases). The goal of this research was to explore the potential of RNase U2 to improve sequence coverage in RNA modification mapping. Mutagenesis of recognition-site residues indicate that work cooperatively to bind ribonucleotides. Glu62, previously thought as a catalytic residue also proved to be more important in binding versus catalysis. Finally, a variant E49A was found to generate long overlapping digestion products leading to a method that can generate 100% sequence coverage of tRNAs using only one RNase. 2019-10-18 English text University of Cincinnati / OhioLINK http://rave.ohiolink.edu/etdc/view?acc_num=ucin156387231304478 http://rave.ohiolink.edu/etdc/view?acc_num=ucin156387231304478 unrestricted This thesis or dissertation is protected by copyright: some rights reserved. It is licensed for use under a Creative Commons license. Specific terms and permissions are available from this document's record in the OhioLINK ETD Center. |
collection |
NDLTD |
language |
English |
sources |
NDLTD |
topic |
Analytical Chemistry RNA Modification Mapping Ribonuclease Mass spectrometry RNase U2 Enzyme |
spellingShingle |
Analytical Chemistry RNA Modification Mapping Ribonuclease Mass spectrometry RNase U2 Enzyme Solivio, Beulah Mae Ann Studying the RNA-Recognition Site of RNase U2 for a More Diverse Bioanalytical Toolbox in RNA Modification Mapping |
author |
Solivio, Beulah Mae Ann |
author_facet |
Solivio, Beulah Mae Ann |
author_sort |
Solivio, Beulah Mae Ann |
title |
Studying the RNA-Recognition Site of RNase U2 for a More Diverse Bioanalytical Toolbox in RNA Modification Mapping |
title_short |
Studying the RNA-Recognition Site of RNase U2 for a More Diverse Bioanalytical Toolbox in RNA Modification Mapping |
title_full |
Studying the RNA-Recognition Site of RNase U2 for a More Diverse Bioanalytical Toolbox in RNA Modification Mapping |
title_fullStr |
Studying the RNA-Recognition Site of RNase U2 for a More Diverse Bioanalytical Toolbox in RNA Modification Mapping |
title_full_unstemmed |
Studying the RNA-Recognition Site of RNase U2 for a More Diverse Bioanalytical Toolbox in RNA Modification Mapping |
title_sort |
studying the rna-recognition site of rnase u2 for a more diverse bioanalytical toolbox in rna modification mapping |
publisher |
University of Cincinnati / OhioLINK |
publishDate |
2019 |
url |
http://rave.ohiolink.edu/etdc/view?acc_num=ucin156387231304478 |
work_keys_str_mv |
AT soliviobeulahmaeann studyingthernarecognitionsiteofrnaseu2foramorediversebioanalyticaltoolboxinrnamodificationmapping |
_version_ |
1719460074047406080 |