Studying the RNA-Recognition Site of RNase U2 for a More Diverse Bioanalytical Toolbox in RNA Modification Mapping

Studying the RNA-Recognition Site of RNase U2 for a More Diverse Bioanalytical Toolbox in RNA Modification Mapping

Bibliographic Details
Main Author: Solivio, Beulah Mae Ann
Language:English
Published: University of Cincinnati / OhioLINK 2019
Subjects:
Analytical Chemistry
RNA Modification Mapping
Ribonuclease
Mass spectrometry
RNase U2
Enzyme
Online Access:http://rave.ohiolink.edu/etdc/view?acc_num=ucin156387231304478
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spelling ndltd-OhioLink-oai-etd.ohiolink.edu-ucin1563872313044782021-08-16T05:10:40Z Studying the RNA-Recognition Site of RNase U2 for a More Diverse Bioanalytical Toolbox in RNA Modification Mapping Solivio, Beulah Mae Ann Analytical Chemistry RNA Modification Mapping Ribonuclease Mass spectrometry RNase U2 Enzyme This dissertation is focused on enhancing the available tools for RNA analysis in terms of ribonucleases (RNases). The goal of this research was to explore the potential of RNase U2 to improve sequence coverage in RNA modification mapping. Mutagenesis of recognition-site residues indicate that work cooperatively to bind ribonucleotides. Glu62, previously thought as a catalytic residue also proved to be more important in binding versus catalysis. Finally, a variant E49A was found to generate long overlapping digestion products leading to a method that can generate 100% sequence coverage of tRNAs using only one RNase. 2019-10-18 English text University of Cincinnati / OhioLINK http://rave.ohiolink.edu/etdc/view?acc_num=ucin156387231304478 http://rave.ohiolink.edu/etdc/view?acc_num=ucin156387231304478 unrestricted This thesis or dissertation is protected by copyright: some rights reserved. It is licensed for use under a Creative Commons license. Specific terms and permissions are available from this document's record in the OhioLINK ETD Center.
collection NDLTD
language English
sources NDLTD
topic Analytical Chemistry
RNA Modification Mapping
Ribonuclease
Mass spectrometry
RNase U2
Enzyme
spellingShingle Analytical Chemistry
RNA Modification Mapping
Ribonuclease
Mass spectrometry
RNase U2
Enzyme
Solivio, Beulah Mae Ann
Studying the RNA-Recognition Site of RNase U2 for a More Diverse Bioanalytical Toolbox in RNA Modification Mapping
author Solivio, Beulah Mae Ann
author_facet Solivio, Beulah Mae Ann
author_sort Solivio, Beulah Mae Ann
title Studying the RNA-Recognition Site of RNase U2 for a More Diverse Bioanalytical Toolbox in RNA Modification Mapping
title_short Studying the RNA-Recognition Site of RNase U2 for a More Diverse Bioanalytical Toolbox in RNA Modification Mapping
title_full Studying the RNA-Recognition Site of RNase U2 for a More Diverse Bioanalytical Toolbox in RNA Modification Mapping
title_fullStr Studying the RNA-Recognition Site of RNase U2 for a More Diverse Bioanalytical Toolbox in RNA Modification Mapping
title_full_unstemmed Studying the RNA-Recognition Site of RNase U2 for a More Diverse Bioanalytical Toolbox in RNA Modification Mapping
title_sort studying the rna-recognition site of rnase u2 for a more diverse bioanalytical toolbox in rna modification mapping
publisher University of Cincinnati / OhioLINK
publishDate 2019
url http://rave.ohiolink.edu/etdc/view?acc_num=ucin156387231304478
work_keys_str_mv AT soliviobeulahmaeann studyingthernarecognitionsiteofrnaseu2foramorediversebioanalyticaltoolboxinrnamodificationmapping
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