A Mobile Healthcare (mHEALTH) System Using Polymer Lab-On-A-Chip With Chemiluminescence Based High-Sensitive Immunoassay For Clinical Diagnostics

A Mobile Healthcare (mHEALTH) System Using Polymer Lab-On-A-Chip With Chemiluminescence Based High-Sensitive Immunoassay For Clinical Diagnostics

Bibliographic Details
Main Author: Ghosh, Sthitodhi
Language:English
Published: University of Cincinnati / OhioLINK 2020
Subjects:
Electrical Engineering
Capillary Microfluidics
Point of care Testing POCT
Lyophilization
Chemiluminescence Immunoassay
Infectious Disease
Mobile Healthcare
Online Access:http://rave.ohiolink.edu/etdc/view?acc_num=ucin1592170905649462
id ndltd-OhioLink-oai-etd.ohiolink.edu-ucin1592170905649462
record_format oai_dc
collection NDLTD
language English
sources NDLTD
topic Electrical Engineering
Capillary Microfluidics
Point of care Testing POCT
Lyophilization
Chemiluminescence Immunoassay
Infectious Disease
Mobile Healthcare
spellingShingle Electrical Engineering
Capillary Microfluidics
Point of care Testing POCT
Lyophilization
Chemiluminescence Immunoassay
Infectious Disease
Mobile Healthcare
Ghosh, Sthitodhi
A Mobile Healthcare (mHEALTH) System Using Polymer Lab-On-A-Chip With Chemiluminescence Based High-Sensitive Immunoassay For Clinical Diagnostics
author Ghosh, Sthitodhi
author_facet Ghosh, Sthitodhi
author_sort Ghosh, Sthitodhi
title A Mobile Healthcare (mHEALTH) System Using Polymer Lab-On-A-Chip With Chemiluminescence Based High-Sensitive Immunoassay For Clinical Diagnostics
title_short A Mobile Healthcare (mHEALTH) System Using Polymer Lab-On-A-Chip With Chemiluminescence Based High-Sensitive Immunoassay For Clinical Diagnostics
title_full A Mobile Healthcare (mHEALTH) System Using Polymer Lab-On-A-Chip With Chemiluminescence Based High-Sensitive Immunoassay For Clinical Diagnostics
title_fullStr A Mobile Healthcare (mHEALTH) System Using Polymer Lab-On-A-Chip With Chemiluminescence Based High-Sensitive Immunoassay For Clinical Diagnostics
title_full_unstemmed A Mobile Healthcare (mHEALTH) System Using Polymer Lab-On-A-Chip With Chemiluminescence Based High-Sensitive Immunoassay For Clinical Diagnostics
title_sort mobile healthcare (mhealth) system using polymer lab-on-a-chip with chemiluminescence based high-sensitive immunoassay for clinical diagnostics
publisher University of Cincinnati / OhioLINK
publishDate 2020
url http://rave.ohiolink.edu/etdc/view?acc_num=ucin1592170905649462
work_keys_str_mv AT ghoshsthitodhi amobilehealthcaremhealthsystemusingpolymerlabonachipwithchemiluminescencebasedhighsensitiveimmunoassayforclinicaldiagnostics
AT ghoshsthitodhi mobilehealthcaremhealthsystemusingpolymerlabonachipwithchemiluminescencebasedhighsensitiveimmunoassayforclinicaldiagnostics
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spelling ndltd-OhioLink-oai-etd.ohiolink.edu-ucin15921709056494622021-10-16T05:25:16Z A Mobile Healthcare (mHEALTH) System Using Polymer Lab-On-A-Chip With Chemiluminescence Based High-Sensitive Immunoassay For Clinical Diagnostics Ghosh, Sthitodhi Electrical Engineering Capillary Microfluidics Point of care Testing POCT Lyophilization Chemiluminescence Immunoassay Infectious Disease Mobile Healthcare The objective of this research work is to develop a functional lab-on-chip (LOC) and a smartphone compatible point-of-care-testing (POCT) platform to quantitatively determine the concentration of a target biomarker in biological fluids for disease diagnostics in resource-limited environment. Majority of deaths related to infectious diseases occur in resource-poor countries having limited access to clinical laboratory facilities and trained personnel. Developing reliable diagnostic tests to be used at the point-of-care can result in earlier disease diagnosis, improved patient treatment, and more efficient outbreak prevention. The realization of an ideal POCT system largely relies on the development of cheap and disposable micro?uidic devices that can be easily integrated to low power electronics with a user-friendly interface.In this research, for the first time, a new microchannel capillary flow assay (MCFA) LOC for high-sensitive chemiluminescence ELISA using on-chip reagent lyophilization has been developed and applied for the detection of malarial biomarker Plasmodium Falciparum Histidine Rich Protein 2 (PfHRP2). This work also reports the design and development of a smartphone based POCT analyzer for detecting chemiluminescent signals from preloaded MCFA LOC. MCFA LOCs were designed for single-step sample loading and capillary liquid transport to initiate chemiluminescence sandwich ELISA in the reaction chambers. A methodology towards lyophilization of chemiluminescent substrate while restoring the substrate functionality in artificial serum was successfully established which paves the way towards developing a sample-to-answer type POCT platform . On sample addition, the functionally designed microchannels with adequately hydrophilic surfaces pull the sample towards the dried reagents and reconstitutes them.The designed microfluidic chip controls sequential arrival of reconstituted reagents in the reaction chamber producing the desired chemiluminescent light which can be correlated to the target biomarker concentration. No requirement of any external flow control and chemiluminescence based detection (which does not require any excitation source) makes the lab chip easily integrable to a smartphone through an attachable/detachable optical analyzer. A smartphone-based portable POCT analyzer was designed, developed and characterized for chemiluminescent detection from the preloaded MCFA LOC. The smartphone based portable analyzer system consists of an android smartphone , a low-level light detection circuit assembled in a 3D printed black box, and the fabricated MCFA LOC. The optical detector detects the chemiluminescence in the spiral reaction chambers of the MCFA chip, amplifies the signal and transmits digital data to the smartphone for processing and display. The smartphone powers the electronic circuit and communicates with it via an USB cable using the USB-OTG protocol. The overall functionality of the integrated system was validated with PfHRP2-spiked serum with results obtained within 20 minutes of sample addition. The standard curve calculated from the smartphone analyzer was comparable to conventional readers with clinically relevant limit of detection. This work thus reports the successful development of a hand-held POCT analyzer for detecting infectious diseases like malaria. The developed MCFA lab chip with the smartphone analyzer can be easily customized and can provide a low cost, low power, portable platform for fast and sensitive detection of various biomarkers for disease diagnostics and prevention. 2020-10-15 English text University of Cincinnati / OhioLINK http://rave.ohiolink.edu/etdc/view?acc_num=ucin1592170905649462 http://rave.ohiolink.edu/etdc/view?acc_num=ucin1592170905649462 unrestricted This thesis or dissertation is protected by copyright: all rights reserved. It may not be copied or redistributed beyond the terms of applicable copyright laws.

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