Epigenetic and Transcriptional Dysregulation in Atopic Dermatitis

Epigenetic and Transcriptional Dysregulation in Atopic Dermatitis

Bibliographic Details
Main Author: Eapen, Amy
Language:English
Published: University of Cincinnati / OhioLINK 2020
Subjects:
Genetics
atopic dermatitis
epigenetics
transcriptional dysregulation
Online Access:http://rave.ohiolink.edu/etdc/view?acc_num=ucin1593170725231765
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spelling ndltd-OhioLink-oai-etd.ohiolink.edu-ucin15931707252317652021-08-03T07:15:21Z Epigenetic and Transcriptional Dysregulation in Atopic Dermatitis Eapen, Amy Genetics atopic dermatitis epigenetics transcriptional dysregulation Atopic dermatitis (AD), the start of the atopic march, is one of the most common skin disorders in children. Immunologically, AD involves skin barrier defects and CD4+ T cells that localize to the skin, producing inflammatory cytokines and amplifying epidermal dysfunction. To understand the differences in activated CD4+ T cells from people with and without AD, we designed a study to measure chromatin openness and gene expression from uniformly activated CD4+ T cells isolated from the peripheral blood. We recruited a total of 14 age-matched moderate-to-severe AD patients and controls for this study. RNA-sequencing of activated CD4+ T cells identified differentially expressed genes. ATAC-sequencing was used to measure genome-wide disease-dependent chromatin openness. Transcription factors whose binding motifs were enriched in an AD-dependent manner were identified. Through ATAC-sequencing motif enrichment analysis, NFkB was identified to be a key transcription factor. Altogether, this thesis project elucidated AD-dependent transcriptional regulation and nominated key TFs as being mechanistically important. 2020-11-05 English text University of Cincinnati / OhioLINK http://rave.ohiolink.edu/etdc/view?acc_num=ucin1593170725231765 http://rave.ohiolink.edu/etdc/view?acc_num=ucin1593170725231765 restricted--full text unavailable until 2022-11-05 This thesis or dissertation is protected by copyright: all rights reserved. It may not be copied or redistributed beyond the terms of applicable copyright laws.
collection NDLTD
language English
sources NDLTD
topic Genetics
atopic dermatitis
epigenetics
transcriptional dysregulation
spellingShingle Genetics
atopic dermatitis
epigenetics
transcriptional dysregulation
Eapen, Amy
Epigenetic and Transcriptional Dysregulation in Atopic Dermatitis
author Eapen, Amy
author_facet Eapen, Amy
author_sort Eapen, Amy
title Epigenetic and Transcriptional Dysregulation in Atopic Dermatitis
title_short Epigenetic and Transcriptional Dysregulation in Atopic Dermatitis
title_full Epigenetic and Transcriptional Dysregulation in Atopic Dermatitis
title_fullStr Epigenetic and Transcriptional Dysregulation in Atopic Dermatitis
title_full_unstemmed Epigenetic and Transcriptional Dysregulation in Atopic Dermatitis
title_sort epigenetic and transcriptional dysregulation in atopic dermatitis
publisher University of Cincinnati / OhioLINK
publishDate 2020
url http://rave.ohiolink.edu/etdc/view?acc_num=ucin1593170725231765
work_keys_str_mv AT eapenamy epigeneticandtranscriptionaldysregulationinatopicdermatitis
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