DIFFERENTIAL GENE EXPRESSION IN PERIPLANETA americana IN RESPONSE TO IMMUNIZATION WITH BEE PHOSPHOLIPASE A2

Bibliographic Details
Main Author:	Clyne, Erin Michele
Language:	English
Published:	University of Cincinnati / OhioLINK 2001
Subjects:	AMERICAN COCKROACH (PERIPLANETA AMERICANA) ADAPTIVE HUMORAL IMMUNITY INSECT IMMUNITY
Online Access:	http://rave.ohiolink.edu/etdc/view?acc_num=ucin997793741

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spelling	ndltd-OhioLink-oai-etd.ohiolink.edu-ucin9977937412021-08-03T06:16:29Z DIFFERENTIAL GENE EXPRESSION IN PERIPLANETA americana IN RESPONSE TO IMMUNIZATION WITH BEE PHOSPHOLIPASE A2 Clyne, Erin Michele AMERICAN COCKROACH (PERIPLANETA AMERICANA) ADAPTIVE HUMORAL IMMUNITY INSECT IMMUNITY The evolutionary relationship of immune systems in invertebrates and vertebrates has caused a controversy in comparative immunology. It is generally accepted that both types of animals have evolved structurally similar non-specific, innate humoral immune defense strategies in a continuous line. However, recent evidence supports a theory that many components of the innate system may have independently evolved immune functions in the two groups of animals. As for adaptive immunity, many scientists argue that this type of advanced system evolved only in the vertebrates. Yet, studies on the American cockroach (Periplaneta americana) in Dr. Richard Karp's lab have led to the discovery of a possible invertebrate-type of adaptive humoral immune response, which may have evolved independently of the vertebrate equivalent. The response in the roaches displayed both specificity and memory after immunizations with a deactivated bee phospholipase A2 (BPA2) antigen and appeared to be mediated by a 102kDa inducible humoral protein factor. To identify genes involved in the response, a differential screening of a cDNA library from BPA2-immunized roaches yielded 16 potential differentially expressed candidate clones. Six of these clones were sequenced and analyzed using BLAST(r) database searches to identify clones with unique sequences or with high identity to sequences of genes with immune or unknown functions. The remaining ten clones have now been subcloned, sequenced, and analyzed using the same criteria. Out of the sixteen, eleven of the clones were identified as unique or with sequence similarity to sequences with unknown or immune functions. Nine of these 11 clones were subjected successfully to mRNA-enriched Northern blots or semi-quantitative duplex RT-PCR in order to confirm their differential expression. The results indicated that only four of the clones were confirmed to have differential expression with one clone potentially up-regulated and three clones possibly down-regulated in the immune response to the BPA2 antigen. These results and the likelihood of finding the last two clones differentially expressed appear to support the possible evolution of adaptive immunity in at least one advanced invertebrate. Yet, only future molecular characterization will allow for the identification of all the genes involved, their functions, and their true evolutionary history. 2001-10-11 English text University of Cincinnati / OhioLINK http://rave.ohiolink.edu/etdc/view?acc_num=ucin997793741 http://rave.ohiolink.edu/etdc/view?acc_num=ucin997793741 restricted--full text not available online This thesis or dissertation is protected by copyright: all rights reserved. It may not be copied or redistributed beyond the terms of applicable copyright laws.
collection	NDLTD
language	English
sources	NDLTD
topic	AMERICAN COCKROACH (PERIPLANETA AMERICANA) ADAPTIVE HUMORAL IMMUNITY INSECT IMMUNITY
spellingShingle	AMERICAN COCKROACH (PERIPLANETA AMERICANA) ADAPTIVE HUMORAL IMMUNITY INSECT IMMUNITY Clyne, Erin Michele DIFFERENTIAL GENE EXPRESSION IN PERIPLANETA americana IN RESPONSE TO IMMUNIZATION WITH BEE PHOSPHOLIPASE A2
author	Clyne, Erin Michele
author_facet	Clyne, Erin Michele
author_sort	Clyne, Erin Michele
title	DIFFERENTIAL GENE EXPRESSION IN PERIPLANETA americana IN RESPONSE TO IMMUNIZATION WITH BEE PHOSPHOLIPASE A2
title_short	DIFFERENTIAL GENE EXPRESSION IN PERIPLANETA americana IN RESPONSE TO IMMUNIZATION WITH BEE PHOSPHOLIPASE A2
title_full	DIFFERENTIAL GENE EXPRESSION IN PERIPLANETA americana IN RESPONSE TO IMMUNIZATION WITH BEE PHOSPHOLIPASE A2
title_fullStr	DIFFERENTIAL GENE EXPRESSION IN PERIPLANETA americana IN RESPONSE TO IMMUNIZATION WITH BEE PHOSPHOLIPASE A2
title_full_unstemmed	DIFFERENTIAL GENE EXPRESSION IN PERIPLANETA americana IN RESPONSE TO IMMUNIZATION WITH BEE PHOSPHOLIPASE A2
title_sort	differential gene expression in periplaneta americana in response to immunization with bee phospholipase a2
publisher	University of Cincinnati / OhioLINK
publishDate	2001
url	http://rave.ohiolink.edu/etdc/view?acc_num=ucin997793741
work_keys_str_mv	AT clyneerinmichele differentialgeneexpressioninperiplanetaamericanainresponsetoimmunizationwithbeephospholipasea2
_version_	1719433840071540736

DIFFERENTIAL GENE EXPRESSION IN PERIPLANETA americana IN RESPONSE TO IMMUNIZATION WITH BEE PHOSPHOLIPASE A2

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