The Effects of SOCS1 and SOCS3 Peptide Mimetics on Macrophage Phagocytosis of Malignant Cells

The Effects of SOCS1 and SOCS3 Peptide Mimetics on Macrophage Phagocytosis of Malignant Cells

Bibliographic Details
Main Author: Madkhali, Tahirah M.
Language:English
Published: Wright State University / OhioLINK 2019
Subjects:
Microbiology
Immunology
SOCS1
SOCS3
Cytokine Signal
Peptide Mimetics
Macrophage
Phagocytosis
Malignant Cells
Calreticulin
Online Access:http://rave.ohiolink.edu/etdc/view?acc_num=wright1557480896176185
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spelling ndltd-OhioLink-oai-etd.ohiolink.edu-wright15574808961761852021-08-03T07:11:12Z The Effects of SOCS1 and SOCS3 Peptide Mimetics on Macrophage Phagocytosis of Malignant Cells Madkhali, Tahirah M. Microbiology Immunology SOCS1 SOCS3 Cytokine Signal Peptide Mimetics Macrophage Phagocytosis Malignant Cells Calreticulin Macrophages are essential phagocytic cells involved in both innate and adaptive immune systems and play vital roles in the host defense and inflammation. Macrophages have a remarkably high capacity to clear unnecessary cellular materials in interstitial environment through a process called “phagocytosis”, which is affected by many factors including suppressors of cytokine signaling (SOCS). SOCSs are a group of intracellular proteins that downregulate the cytokine signals involved in various JAK/STAT pathways through a negative feedback loop. This study focuses on investigating the effects of SOCS1 and SOCS3 on the phagocytic ability of RAW 264.7 macrophages polarized into M2a with IL-4/IL-13 and into M2c with IL-10 to clear fluorescently marked Neuro-2a malignant cells. Calreticulin (CRT) is one of the cellular signals, exposed on the surface of malignant cells, that play vital roles in controlling the phagocytosis process mediate by phagocytes including macrophages. Treatment of M2a (IL-4/IL-13) and M2c macrophages with SOCS1 peptide mimetics had no significant effects on M2a and M2c-mediated phagocytosis of CFSE-stained N2a cells compared with M2a (IL-4/IL-13) and M2c cells without SOCS1 peptide mimetic treatment, while it slightly increased the M2a (IL-4/IL-13), but not M2c, macrophage-mediated phagocytosis of N2a cells that had their calreticulin receptors blocked. When N2a cells were blocked with anti-CRT antibody, M2a and M2c-mediated phagocytosis was decreased. Interestingly, treatment of M2c with SOCS3 peptide mimetics coincided with blockage of CRT signals on N2a cells caused a significant increase in the phagocytosis of N2a cells mediated by M2c polarized macrophages. These data help to get a deeper understanding of macrophage functions in cleaning the microenvironment from apoptotic and malignant cells and even wound healing. Overall, this study sheds more light on how SOCS1/ SOCS3 can be used along with manipulation of “eat-me” signals exposed on malignant and apoptotic cells to increase the clearance rate of malignant cells and decrease the tumor survival. 2019-05-14 English text Wright State University / OhioLINK http://rave.ohiolink.edu/etdc/view?acc_num=wright1557480896176185 http://rave.ohiolink.edu/etdc/view?acc_num=wright1557480896176185 unrestricted This thesis or dissertation is protected by copyright: all rights reserved. It may not be copied or redistributed beyond the terms of applicable copyright laws.
collection NDLTD
language English
sources NDLTD
topic Microbiology
Immunology
SOCS1
SOCS3
Cytokine Signal
Peptide Mimetics
Macrophage
Phagocytosis
Malignant Cells
Calreticulin
spellingShingle Microbiology
Immunology
SOCS1
SOCS3
Cytokine Signal
Peptide Mimetics
Macrophage
Phagocytosis
Malignant Cells
Calreticulin
Madkhali, Tahirah M.
The Effects of SOCS1 and SOCS3 Peptide Mimetics on Macrophage Phagocytosis of Malignant Cells
author Madkhali, Tahirah M.
author_facet Madkhali, Tahirah M.
author_sort Madkhali, Tahirah M.
title The Effects of SOCS1 and SOCS3 Peptide Mimetics on Macrophage Phagocytosis of Malignant Cells
title_short The Effects of SOCS1 and SOCS3 Peptide Mimetics on Macrophage Phagocytosis of Malignant Cells
title_full The Effects of SOCS1 and SOCS3 Peptide Mimetics on Macrophage Phagocytosis of Malignant Cells
title_fullStr The Effects of SOCS1 and SOCS3 Peptide Mimetics on Macrophage Phagocytosis of Malignant Cells
title_full_unstemmed The Effects of SOCS1 and SOCS3 Peptide Mimetics on Macrophage Phagocytosis of Malignant Cells
title_sort effects of socs1 and socs3 peptide mimetics on macrophage phagocytosis of malignant cells
publisher Wright State University / OhioLINK
publishDate 2019
url http://rave.ohiolink.edu/etdc/view?acc_num=wright1557480896176185
work_keys_str_mv AT madkhalitahirahm theeffectsofsocs1andsocs3peptidemimeticsonmacrophagephagocytosisofmalignantcells
AT madkhalitahirahm effectsofsocs1andsocs3peptidemimeticsonmacrophagephagocytosisofmalignantcells
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