Generation of Human Aldose Reductase Mutants of Cys298
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Youngstown State University / OhioLINK
2015
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| Online Access: | http://rave.ohiolink.edu/etdc/view?acc_num=ysu1443087374 |
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ndltd-OhioLink-oai-etd.ohiolink.edu-ysu14430873742021-08-03T06:33:29Z Generation of Human Aldose Reductase Mutants of Cys298 Oder, Daniel O. Biochemistry Biology Chemistry Molecular Biology Human aldose reductase Cys298 mutants Hyperglycemic tissue damage Human aldose reductase (hAR) uses the oxidation of NADPH to NADP<sup>+</sup> in order to catalyze the reduction of aldehydes to their corresponding alcohols. This enzyme is important because of its role in the polyol pathway and hyperglycemia-induced tissue damage. Several studies have shown that Cys298 has a role in the activation of hAR to its oxidized form. Even so, though it is not a catalytic residue the mechanism of hAR activation is not yet clearly understood. Structure, function and kinetic studies of Cys298 mutants will further our understanding of the role of this residue in substrate binding and interaction. This thesis project forms the foundation for future investigations since it provides an efficient, reproducible and reliable protein expression and protein purification system for three Cys298 mutants: Cys298Ala, Cys298Asp, and Cys298Gly. 2015-09-24 English text Youngstown State University / OhioLINK http://rave.ohiolink.edu/etdc/view?acc_num=ysu1443087374 http://rave.ohiolink.edu/etdc/view?acc_num=ysu1443087374 unrestricted This thesis or dissertation is protected by copyright: all rights reserved. It may not be copied or redistributed beyond the terms of applicable copyright laws. |
| collection |
NDLTD |
| language |
English |
| sources |
NDLTD |
| topic |
Biochemistry Biology Chemistry Molecular Biology Human aldose reductase Cys298 mutants Hyperglycemic tissue damage |
| spellingShingle |
Biochemistry Biology Chemistry Molecular Biology Human aldose reductase Cys298 mutants Hyperglycemic tissue damage Oder, Daniel O. Generation of Human Aldose Reductase Mutants of Cys298 |
| author |
Oder, Daniel O. |
| author_facet |
Oder, Daniel O. |
| author_sort |
Oder, Daniel O. |
| title |
Generation of Human Aldose Reductase Mutants of Cys298 |
| title_short |
Generation of Human Aldose Reductase Mutants of Cys298 |
| title_full |
Generation of Human Aldose Reductase Mutants of Cys298 |
| title_fullStr |
Generation of Human Aldose Reductase Mutants of Cys298 |
| title_full_unstemmed |
Generation of Human Aldose Reductase Mutants of Cys298 |
| title_sort |
generation of human aldose reductase mutants of cys298 |
| publisher |
Youngstown State University / OhioLINK |
| publishDate |
2015 |
| url |
http://rave.ohiolink.edu/etdc/view?acc_num=ysu1443087374 |
| work_keys_str_mv |
AT oderdanielo generationofhumanaldosereductasemutantsofcys298 |
| _version_ |
1719439217662099456 |