Selection of Phage Displaying Peptides Specific for Staphylococcus Aureus
| Main Author: | |
|---|---|
| Language: | English |
| Published: |
Youngstown State University / OhioLINK
2021
|
| Subjects: | |
| Online Access: | http://rave.ohiolink.edu/etdc/view?acc_num=ysu1619516455248529 |
| id |
ndltd-OhioLink-oai-etd.ohiolink.edu-ysu1619516455248529 |
|---|---|
| record_format |
oai_dc |
| spelling |
ndltd-OhioLink-oai-etd.ohiolink.edu-ysu16195164552485292021-08-03T07:17:18Z Selection of Phage Displaying Peptides Specific for Staphylococcus Aureus Gadzekpo, Isaac Kwabena Biology Staphylococcus aureus Phage display peptide Staphylococcus aureus is a notorious Gram positive bacterium that has been implicated in causing hospital-acquired and community-associated infections. The goal of this project was to retest previously selected S. aureus-specific phage clones from the phage library PhD-7 (New England BioLabs) and identify phage with a peptide that is able to bind to the capsular polysaccharide of S. aureus. The phage clones were amplified and PEG-purified prior to testing for specificity by performing ELISAs and sequencing. Whole cell bacterium ELISA using PEG-purified phage MMT5-2, 4, 5, 6 and 7 showed that all phage clones tested bound preferentially to S. aureus, except MMT5-7. Using purified carbohydrate as antigen, phage clone MMT5-4 showed high background but preferential binding to the capsule of S. aureus, while MMT5-6 showed non-specific binding. MMT5-2, 4, 5, 6 and 7 were selected for sequencing and MMT5-4 DNA sequence was translated into peptide sequence. The sequence revealed that the peptide displayed by the phage was SARXLLK and that the peptide was positively charged and hydrophobic in nature. Sequencing of our selected phage clone MMT5-4 agreed to Rao’s work with respect to the polar nature and hydrophobicity of the amino acid. However, our peptide did not show the LQX motif, as reported by Rao and coworkers. Nevertheless, it did show binding specificity against S. aureus as a whole cell bacterium, like Rao’s peptide and most importantly it showed specific binding to the capsule of S. aureus, type 5. This work is the first to report a peptide specific for S. aureus capsular carbohydrate. In future studies we hope to combine a toxin to the peptide to eradicate S. aureus infections. 2021-05-06 English text Youngstown State University / OhioLINK http://rave.ohiolink.edu/etdc/view?acc_num=ysu1619516455248529 http://rave.ohiolink.edu/etdc/view?acc_num=ysu1619516455248529 unrestricted This thesis or dissertation is protected by copyright: all rights reserved. It may not be copied or redistributed beyond the terms of applicable copyright laws. |
| collection |
NDLTD |
| language |
English |
| sources |
NDLTD |
| topic |
Biology Staphylococcus aureus Phage display peptide |
| spellingShingle |
Biology Staphylococcus aureus Phage display peptide Gadzekpo, Isaac Kwabena Selection of Phage Displaying Peptides Specific for Staphylococcus Aureus |
| author |
Gadzekpo, Isaac Kwabena |
| author_facet |
Gadzekpo, Isaac Kwabena |
| author_sort |
Gadzekpo, Isaac Kwabena |
| title |
Selection of Phage Displaying Peptides Specific for Staphylococcus Aureus |
| title_short |
Selection of Phage Displaying Peptides Specific for Staphylococcus Aureus |
| title_full |
Selection of Phage Displaying Peptides Specific for Staphylococcus Aureus |
| title_fullStr |
Selection of Phage Displaying Peptides Specific for Staphylococcus Aureus |
| title_full_unstemmed |
Selection of Phage Displaying Peptides Specific for Staphylococcus Aureus |
| title_sort |
selection of phage displaying peptides specific for staphylococcus aureus |
| publisher |
Youngstown State University / OhioLINK |
| publishDate |
2021 |
| url |
http://rave.ohiolink.edu/etdc/view?acc_num=ysu1619516455248529 |
| work_keys_str_mv |
AT gadzekpoisaackwabena selectionofphagedisplayingpeptidesspecificforstaphylococcusaureus |
| _version_ |
1719458625291812864 |