Lineage II of Southeast Asian/American DENV-2 is Associated with a Severe Dengue Outbreak in the Peruvian Amazon
During 2010 and 2011, the Loreto region of Peru experienced a dengue outbreak of unprecedented magnitude and severity for the region. This outbreak coincided with the reappearance of dengue virus-2 (DENV-2) in Loreto after almost 8 years. Whole-genome sequence indicated that DENV-2 from the outbre...
Main Authors: | , , , , , , , , , , , , , |
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Format: | Article |
Language: | English |
Published: |
The American Society of Tropical Medicine and Hygiene
2014
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Subjects: | |
Online Access: | http://hdl.handle.net/10757/605309 http://repositorioacademico.upc.edu.pe/upc/handle/10757/605309 |
Summary: | During 2010 and 2011, the Loreto region of Peru experienced a dengue outbreak of unprecedented
magnitude and severity for the region. This outbreak coincided with the reappearance of dengue virus-2 (DENV-2) in
Loreto after almost 8 years. Whole-genome sequence indicated that DENV-2 from the outbreak belonged to lineage II
of the southeast Asian/American genotype and was most closely related to viruses circulating in Brazil during 2007 and
2008, whereas DENV-2 previously circulating in Loreto grouped with lineage I (DENV-2 strains circulating in South
America since 1990). One amino acid substitution (NS5 A811V) in the 2010 and 2011 isolates resulted from positive
selection. However, the 2010 and 2011 DENV-2 did not replicate to higher titers in monocyte-derived dendritic cells and
did not infect or disseminate in a higher proportion of Aedes aegypti than DENV-2 isolates previously circulating in Loreto.
These results suggest that factors other than enhanced viral replication played a role in the severity of this outbreak. |
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