Microfluidic Interfaces for Mass Spectrometry: Methods and Applications
Since the introduction of electrospray ionization (ESI) and matrix assisted laser desorption ionization (MALDI), there has been an unprecedented growth of biomolecule analysis using mass spectrometry (MS). One of the most popular applications for mass spectrometry is the field of proteomics, which h...
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ndltd-TORONTO-oai-tspace.library.utoronto.ca-1807-319822013-04-19T19:56:41ZMicrofluidic Interfaces for Mass Spectrometry: Methods and ApplicationsYang, HaoDigital MicrofluidicsMass SpectrometryMicrochannelElectrospray ionizationInterfacesmatrix assisted laser desorption ionization0486Since the introduction of electrospray ionization (ESI) and matrix assisted laser desorption ionization (MALDI), there has been an unprecedented growth of biomolecule analysis using mass spectrometry (MS). One of the most popular applications for mass spectrometry is the field of proteomics, which has emerged as the next scientific challenge in the post-genome era. One critical step in proteomic analysis is sample preparation, a major bottleneck that is attributed to many time consuming and labor-intensive steps involved. Microfluidics can play an important role in proteome sample preparation due to its ability to handle small volumes of sample and reagent, and its capability to integrate multiple processes on a single chip with the potential for high-throughput analysis. However, to utilize microfluidic systems for proteome analysis, an efficient interface between microfluidic chip and mass spectrometry is required. This thesis presents several methods for coupling of microfluidic chips with ESI-MS and MALDIMS. III Three microfluidic-ESI interfaces were developed. The first interface involves fabricating a polymer based microchannel at the rectangular corners of the glass substrates using a single photolithography step. The second interface was build upon the previous interface in which a digital microfluidic platform was integrated with the microchannel in a “top-down” format. The integrated microfluidic system was used for inline quantification of amino acids in dried blood spots that have been processed by digital microfluidics. The third interface was formed by sandwiching a pulled glass capillary emitter between two digital microfluidic substrates. This method is a simpler and more direct coupling of digital microfluidics with ESI-MS as compared to the method used for second interface. Finally, a strategy using a removable plastic “skin” was developed to interface digital microfluidics with MALDI-MS for offline sample analysis. We demonstrated the utility of this format by implementing on-chip protein digestion on immobilized enzyme depots.Wheeler, Aaron2011-112012-01-12T17:59:17ZNO_RESTRICTION2012-01-12T17:59:17Z2012-01-12Thesishttp://hdl.handle.net/1807/31982en_ca |
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Digital Microfluidics Mass Spectrometry Microchannel Electrospray ionization Interfaces matrix assisted laser desorption ionization 0486 |
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Digital Microfluidics Mass Spectrometry Microchannel Electrospray ionization Interfaces matrix assisted laser desorption ionization 0486 Yang, Hao Microfluidic Interfaces for Mass Spectrometry: Methods and Applications |
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Since the introduction of electrospray ionization (ESI) and matrix assisted laser
desorption ionization (MALDI), there has been an unprecedented growth of biomolecule analysis using mass spectrometry (MS). One of the most popular applications for mass spectrometry is the field of proteomics, which has emerged as the next scientific challenge in the post-genome era. One critical step in proteomic analysis is sample preparation, a major bottleneck that is attributed to many time consuming and labor-intensive steps involved. Microfluidics can play an important role in proteome sample preparation due to its ability to handle small volumes of sample and reagent, and its capability to integrate multiple processes on a single chip with the
potential for high-throughput analysis. However, to utilize microfluidic systems for proteome analysis, an efficient interface between microfluidic chip and mass spectrometry is required. This thesis presents several methods for coupling of microfluidic chips with ESI-MS and MALDIMS.
III Three microfluidic-ESI interfaces were developed. The first interface involves fabricating
a polymer based microchannel at the rectangular corners of the glass substrates using a single
photolithography step. The second interface was build upon the previous interface in which a
digital microfluidic platform was integrated with the microchannel in a “top-down” format. The integrated microfluidic system was used for inline quantification of amino acids in dried blood spots that have been processed by digital microfluidics. The third interface was formed by sandwiching a pulled glass capillary emitter between two digital microfluidic substrates. This
method is a simpler and more direct coupling of digital microfluidics with ESI-MS as compared to the method used for second interface. Finally, a strategy using a removable plastic “skin” was developed to interface digital microfluidics with MALDI-MS for offline sample analysis. We
demonstrated the utility of this format by implementing on-chip protein digestion on
immobilized enzyme depots. |
author2 |
Wheeler, Aaron |
author_facet |
Wheeler, Aaron Yang, Hao |
author |
Yang, Hao |
author_sort |
Yang, Hao |
title |
Microfluidic Interfaces for Mass Spectrometry: Methods and Applications |
title_short |
Microfluidic Interfaces for Mass Spectrometry: Methods and Applications |
title_full |
Microfluidic Interfaces for Mass Spectrometry: Methods and Applications |
title_fullStr |
Microfluidic Interfaces for Mass Spectrometry: Methods and Applications |
title_full_unstemmed |
Microfluidic Interfaces for Mass Spectrometry: Methods and Applications |
title_sort |
microfluidic interfaces for mass spectrometry: methods and applications |
publishDate |
2011 |
url |
http://hdl.handle.net/1807/31982 |
work_keys_str_mv |
AT yanghao microfluidicinterfacesformassspectrometrymethodsandapplications |
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1716582160594370560 |