The Transfer of Ethyl Glucuronide in the Dually Perfused Ex Vivo Placental Perfusion Model: Implications for Alcohol Screening during Pregnancy
Alcohol consumption during pregnancy can lead to Fetal Alcohol Spectrum Disorder, and because maternal self-reports are often unreliable, a biomarker of alcohol use during pregnancy is needed to accurately determine fetal exposure. Ethyl glucuronide (EtG) is a direct metabolite of ethanol that has b...
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ndltd-TORONTO-oai-tspace.library.utoronto.ca-1807-334342013-04-19T20:03:21ZThe Transfer of Ethyl Glucuronide in the Dually Perfused Ex Vivo Placental Perfusion Model: Implications for Alcohol Screening during PregnancyMatlow, Jeremyethyl glucuronideplacenta0419Alcohol consumption during pregnancy can lead to Fetal Alcohol Spectrum Disorder, and because maternal self-reports are often unreliable, a biomarker of alcohol use during pregnancy is needed to accurately determine fetal exposure. Ethyl glucuronide (EtG) is a direct metabolite of ethanol that has been detected in the meconium of infants born to mothers who consumed alcohol during pregnancy. In the current study, a method was developed and validated for EtG detection in placental perfusate and tissue using gas chromatography-mass spectrometry. Subsequently, the ex vivo human placental perfusion model was used to investigate whether EtG crosses the human placenta. The validated GC-MS method showed sufficient sensitivity in detecting EtG in placental perfusate and tissue. EtG crossed the placenta slowly and transfer was incomplete after 3 hours of perfusion. EtG appears to cross the human placenta and, hence, to represent both maternal and fetal exposure to alcohol.Koren, Gideon2012-112012-11-22T16:58:10ZNO_RESTRICTION2012-11-22T16:58:10Z2012-11-22Thesishttp://hdl.handle.net/1807/33434en_ca |
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en_ca |
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ethyl glucuronide placenta 0419 |
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ethyl glucuronide placenta 0419 Matlow, Jeremy The Transfer of Ethyl Glucuronide in the Dually Perfused Ex Vivo Placental Perfusion Model: Implications for Alcohol Screening during Pregnancy |
description |
Alcohol consumption during pregnancy can lead to Fetal Alcohol Spectrum Disorder,
and because maternal self-reports are often unreliable, a biomarker of alcohol use during
pregnancy is needed to accurately determine fetal exposure. Ethyl glucuronide (EtG) is a
direct metabolite of ethanol that has been detected in the meconium of infants born to mothers
who consumed alcohol during pregnancy. In the current study, a method was developed and
validated for EtG detection in placental perfusate and tissue using gas chromatography-mass
spectrometry. Subsequently, the ex vivo human placental perfusion model was used to
investigate whether EtG crosses the human placenta. The validated GC-MS method showed
sufficient sensitivity in detecting EtG in placental perfusate and tissue. EtG crossed the
placenta slowly and transfer was incomplete after 3 hours of perfusion. EtG appears to cross
the human placenta and, hence, to represent both maternal and fetal exposure to alcohol. |
author2 |
Koren, Gideon |
author_facet |
Koren, Gideon Matlow, Jeremy |
author |
Matlow, Jeremy |
author_sort |
Matlow, Jeremy |
title |
The Transfer of Ethyl Glucuronide in the Dually Perfused Ex Vivo Placental Perfusion Model: Implications for Alcohol Screening during Pregnancy |
title_short |
The Transfer of Ethyl Glucuronide in the Dually Perfused Ex Vivo Placental Perfusion Model: Implications for Alcohol Screening during Pregnancy |
title_full |
The Transfer of Ethyl Glucuronide in the Dually Perfused Ex Vivo Placental Perfusion Model: Implications for Alcohol Screening during Pregnancy |
title_fullStr |
The Transfer of Ethyl Glucuronide in the Dually Perfused Ex Vivo Placental Perfusion Model: Implications for Alcohol Screening during Pregnancy |
title_full_unstemmed |
The Transfer of Ethyl Glucuronide in the Dually Perfused Ex Vivo Placental Perfusion Model: Implications for Alcohol Screening during Pregnancy |
title_sort |
transfer of ethyl glucuronide in the dually perfused ex vivo placental perfusion model: implications for alcohol screening during pregnancy |
publishDate |
2012 |
url |
http://hdl.handle.net/1807/33434 |
work_keys_str_mv |
AT matlowjeremy thetransferofethylglucuronideintheduallyperfusedexvivoplacentalperfusionmodelimplicationsforalcoholscreeningduringpregnancy AT matlowjeremy transferofethylglucuronideintheduallyperfusedexvivoplacentalperfusionmodelimplicationsforalcoholscreeningduringpregnancy |
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1716582635706253312 |