水稻白葉枯病利用噬菌體作生物防治之研究

• Main Author: 曾義雄
• Other Authors: 郭宗德
• Format: Others
• Language: en_US
• Published: 1970
• Online Access: http://ndltd.ncl.edu.tw/handle/19690276810378655611

碩士 === 文化大學 === 農學系 === 58 === For the preliminary information of the biological control of bacterial leaf blight of rice plant with bacteriophage Xf, the stability of the phage and the relationship between environment and phage infection were studied. Fillamentous bacteriophage Xf from Xanthomonas oryzae was quite stable in various conditions. It could be stored in Potato Sugar medium, phosphate buffer or distilled water at 40C for four months without affecting its infectivity. It could also be detected after having aprayed on the leaves of rice plants or in the field water for one month. There were 17 sites of attachment for each host bacterium. It is much larger than that of filamentous bacteriophage of Escherichia coli. Variety of ions on the solution exert different influsence on adsorption rate. The adsorption rates in ions used in this investigation were in the order: NH4+>Mg++>Na+>Ca++>H2O. The adsorption did not affected by the treatment of host bacteria with chloramphenicol. Although chloramphenicol stopped the growth of host bacterium, the adsorption was normal. This implied that the adsorption was not dependent on prtein synthesis, but on the native state of the bacterial cell walls. Heat-killed bacterial cell did not adsorb any infective phage Xf. It appeared that heat treatment destoryed the structure of adsorption sites. This property was completely different from tadpole-shaped bacteriophages.