Studies of Proteins in Porcine Seminal Plasma
博士 === 高雄醫學院 === 醫學研究所 === 81 === Two sperm motility inhibitors (SMI1 and SMI2) were purified from porcine seminal plasma with high performance liquidromatography (HPLC). Their molecular weights are about 15,000 as estimated by SDS-polyactylamide gel elec...
| Main Authors: | , |
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| Other Authors: | |
| Format: | Others |
| Language: | zh-TW |
| Published: |
1993
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| Online Access: | http://ndltd.ncl.edu.tw/handle/37126055094512821968 |
| Summary: | 博士 === 高雄醫學院 === 醫學研究所 === 81 === Two sperm motility inhibitors (SMI1 and SMI2) were purified
from porcine seminal plasma with high performance
liquidromatography (HPLC). Their molecular weights are about
15,000 as estimated by SDS-polyactylamide gel electrophoresis
(SDS- PAGE). Both of them decreased the percentage of motile
spermato- zoa in a dose-dependent manner. The inhibitory effect
can beolished by addition of the porcine follicular fluid. Both
SMI1 and SMI2 have similar amino acid composition, suggesting
that they may be structurally related. They also have
noncompetitive inhibitory effect on chymotrypsin with the Ki
aroune 0.1 uM. And since the FITC-labeled SMI1 and SMI2 binded
to the flagellum ofe active sperm, we might say that the
functional receptor sites is on the flagellum.
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