Inhibition of tonoplast ATPase from etiolated mung bean seedlings by N-bromosucciminide and 2-Hydroxy -5-nitrobenzyl bromide
碩士 === 國立清華大學 === 輻射生物研究所 === 81 === Two modifying reagent, N-bromosuccinimide (NBS) an d 2-hydroxy-5-nitrobenzyl bromide (HNBB), could inhibi t the hydrolysis activity of tonoplast ATPase from eti olated mung bean. Under our condition, HNB...
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ndltd-TW-081NTHU04960102016-07-20T04:11:48Z http://ndltd.ncl.edu.tw/handle/02020587506110569882 Inhibition of tonoplast ATPase from etiolated mung bean seedlings by N-bromosucciminide and 2-Hydroxy -5-nitrobenzyl bromide 以化學修飾法抑制從白化綠豆分離出的腺核甘三磷酸水解酵素 May-Whei Lin 林美慧 碩士 國立清華大學 輻射生物研究所 81 Two modifying reagent, N-bromosuccinimide (NBS) an d 2-hydroxy-5-nitrobenzyl bromide (HNBB), could inhibi t the hydrolysis activity of tonoplast ATPase from eti olated mung bean. Under our condition, HNBB is specifi c for the modification of tryptophan, while NBS could react with several residues besides tryptophan. the in activation of ATPase by HNBB was concentration- and ti me-dependent. From the semilogarithmic plot of time co urse of the inactivation, the reaction order of inacti vation was calculated as 0.973, indicating that at lea st one tryptophan was esstential to the enzymatic acti vity of V-ATPase. Kinetic analysis shows that Km but n ot Vmax of ATPase was changed by NBS, while the Vmax b ut not Km of the enzyme was decreased by HNBB. ATP and its analogs could protect ATPase against NBS but not H NBB, implying that modification sites of NBS and HNBB are not same. Rong-Long Pan 潘榮隆 學位論文 ; thesis 50 zh-TW |
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碩士 === 國立清華大學 === 輻射生物研究所 === 81 === Two modifying reagent, N-bromosuccinimide (NBS) an d
2-hydroxy-5-nitrobenzyl bromide (HNBB), could inhibi t the
hydrolysis activity of tonoplast ATPase from eti olated mung
bean. Under our condition, HNBB is specifi c for the
modification of tryptophan, while NBS could react with several
residues besides tryptophan. the in activation of ATPase by
HNBB was concentration- and ti me-dependent. From the
semilogarithmic plot of time co urse of the inactivation, the
reaction order of inacti vation was calculated as 0.973,
indicating that at lea st one tryptophan was esstential to the
enzymatic acti vity of V-ATPase. Kinetic analysis shows that Km
but n ot Vmax of ATPase was changed by NBS, while the Vmax b ut
not Km of the enzyme was decreased by HNBB. ATP and its analogs
could protect ATPase against NBS but not H NBB, implying that
modification sites of NBS and HNBB are not same.
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author2 |
Rong-Long Pan |
author_facet |
Rong-Long Pan May-Whei Lin 林美慧 |
author |
May-Whei Lin 林美慧 |
spellingShingle |
May-Whei Lin 林美慧 Inhibition of tonoplast ATPase from etiolated mung bean seedlings by N-bromosucciminide and 2-Hydroxy -5-nitrobenzyl bromide |
author_sort |
May-Whei Lin |
title |
Inhibition of tonoplast ATPase from etiolated mung bean seedlings by N-bromosucciminide and 2-Hydroxy -5-nitrobenzyl bromide |
title_short |
Inhibition of tonoplast ATPase from etiolated mung bean seedlings by N-bromosucciminide and 2-Hydroxy -5-nitrobenzyl bromide |
title_full |
Inhibition of tonoplast ATPase from etiolated mung bean seedlings by N-bromosucciminide and 2-Hydroxy -5-nitrobenzyl bromide |
title_fullStr |
Inhibition of tonoplast ATPase from etiolated mung bean seedlings by N-bromosucciminide and 2-Hydroxy -5-nitrobenzyl bromide |
title_full_unstemmed |
Inhibition of tonoplast ATPase from etiolated mung bean seedlings by N-bromosucciminide and 2-Hydroxy -5-nitrobenzyl bromide |
title_sort |
inhibition of tonoplast atpase from etiolated mung bean seedlings by n-bromosucciminide and 2-hydroxy -5-nitrobenzyl bromide |
url |
http://ndltd.ncl.edu.tw/handle/02020587506110569882 |
work_keys_str_mv |
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