A Mathematical Simulation of the Proliferation of Anchorage- Dependent Mammalian Cells
碩士 === 國立臺灣大學 === 化學工程研究所 === 81 === The growth of anchorage-dependent mammalian cells on solid surfaces is investigated. It is assumed that for cells within a critical distance, a microenvironment is formed. If the concen- tration o...
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| Format: | Others |
| Language: | zh-TW |
| Published: |
1993
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| Online Access: | http://ndltd.ncl.edu.tw/handle/60804521481509950541 |
| Summary: | 碩士 === 國立臺灣大學 === 化學工程研究所 === 81 === The growth of anchorage-dependent mammalian cells on solid
surfaces is investigated. It is assumed that for cells within
a critical distance, a microenvironment is formed. If the
concen- tration of an autocrine growth factor in this
microenvironment is greater than a threshold value,
mitogentic reactions occur. It has been shown that the
critical distance is on the order of several cell
diameters. The Monte Carlo simulation is adopted for the
description of the kinetic behavior of the phenomenon under
consideration. In particular, the effects of inoculum
density and contact inhibition on cell growth are
examined. These effects are extremely difficult to be modeled
through the conventional mathematical methods. The cell growth
model proposed in the present study provides a realistic
explanation for the specific nature of the growth of
anchorage-dependent mammalian cells. The growth of human
fibroblast IMR-90 is conducted exper- imentally. The result
obtained is qualitatively in good agreement with the analytical
result.
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