| Summary: | 碩士 === 國立臺灣大學 === 植物病蟲害學系 === 81 === Large quantity of double and single-stranded（ds and ss）
viral DNAs have been purified by using an improved purification
method .Compared with viral genome（ss DNA） and hybridization
analysis ,it is proved that the ss DNA is the viral DNAs
contain both linear and circular form. Two ds viral DNAs
have been cloned. Sequencing analysis of the ds DNA clones
revealed that the viral ds DNA is circular with a size of 1094
bp. The sequence is part- ialy homologous to the genome of
coconut foliar decay virus 1990）and the genome of BBTV
published by Dale（1993）,but both sequence homology are not
significant.Several ss viral DNAs were also cloned. DNA
hybridization tests revealed that there were several forms
of viral genome with different degrees of sequence
homology. I conclude from these results that BBTV is a new
group of circular ss DNA virus, the ds DNA may be the
replication intermediate of viral genome ,and the virus
contains at least 3 DNA components.51
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