Cloning and Molecular analysis of Banana bunchy top virus

• Main Authors: Yeh, Hsin-Hung, 葉信宏
• Other Authors: Su, Hong-Ji
• Format: Others
• Language: zh-TW
• Published: 1993
• Online Access: http://ndltd.ncl.edu.tw/handle/29210634628985811488

碩士 === 國立臺灣大學 === 植物病蟲害學系 === 81 === Large quantity of double and single-stranded（ds and ss） viral DNAs have been purified by using an improved purification method .Compared with viral genome（ss DNA） and hybridization analysis ,it is proved that the ss DNA is the viral DNAs contain both linear and circular form. Two ds viral DNAs have been cloned. Sequencing analysis of the ds DNA clones revealed that the viral ds DNA is circular with a size of 1094 bp. The sequence is part- ialy homologous to the genome of coconut foliar decay virus 1990）and the genome of BBTV published by Dale（1993）,but both sequence homology are not significant.Several ss viral DNAs were also cloned. DNA hybridization tests revealed that there were several forms of viral genome with different degrees of sequence homology. I conclude from these results that BBTV is a new group of circular ss DNA virus, the ds DNA may be the replication intermediate of viral genome ,and the virus contains at least 3 DNA components.51