| Summary: | 碩士 === 國立中興大學 === 獸醫學系 === 82 === Infectious bovine rhinotracheitis virus (IBRV),an
alphaherpesvir us, is a causative agent of infectious bovine
rhinotracheitis (IBR) in cattle. The IBRV synthesizes at least
seven viral enve lope glycoproteins and four of them have been
characterized and named gI,gII.gIII and gIV.Among the four
glycoproteins,gI,gIII and gIV are the major target antigens
involved in virus neutra lization, therefore, they are used as
the subunit vaccine against the infection of IBRV.Previously
our labortary has cloned IBRV (Yunlin strain)glycoprotein gIII
and gIV genes. For the purpose of future study on genetic
structure and development of sudunit vaccine of IBRV it is
essential to proceed the mole cular cloningof IBRV glycoprotein
gI gene. In this experiment, A 12kb BamH I/Hind III fragment of
IBRV (Yunlin strain) DNA was cloned and identified A clone
pBR322A contained gI gene ident ified by polymerase chain
reaction (PCR) of which the primers were desigend according to
IBRV Cooper strain gI gene sequence. A Hpa I/Kpn I fragment of
pBR322A DNA was sudcloned and identi fied. Asubclone pGI-01 of
3.8kd contained gI gene was obtained by restriction mapping and
sequencing. The sequences with 300 base pair long have been
sequenced and included translation start site AUG. The sequence
region was 100%homologous to IBRV Cooper strain. The results
suggested that IBRV (Yunlin strain) glycoprotein gI gene has
been cloned.
|
|---|
