Analysis of Glycoprotein H Gene Sequences of Pseudorabies Virus (PRV) TNL Strain

• Main Authors: Kuo, Shu Ting, 郭舒亭
• Other Authors: Chang, Tien Jye
• Format: Others
• Language: zh-TW
• Published: 1994
• Online Access: http://ndltd.ncl.edu.tw/handle/40645641417794619264

碩士 === 國立中興大學 === 獸醫學系 === 82 === Pseudorabies virus (PRV), a member of Alphaherpes- virinae, is the causative agent of pseudorabies (or Aujeszky's disease). There are at least eight glyco- protein species in PRV. Among these glycoproteins, the glycoprotein H (gH) is the one which was found right before glycoprotein L. It is an essential glycoprotein for virus replication and a protein which induces neutralizing antibody. In this thesis, the nucleotide sequence of gH gene of Taiwan PRV strain, TNL strain, were determined. At first, the putative gH gene was found in the BamHI 11 and 17 fragments of TNL strain as determined by DNA sequencing and southern hybridization. The complete gH gene was cloned by ligation of these two fragments. The resulting clone was used for subsequent subcloning and transcriptional analysis. The gH gene contains 2,058 bps. Two conserved promoter sequence, containing a TATA box and a CAT box, was found upstream of the gene. A potential polyadenylation signal (5'-AATAAA-3) was found at 3' end of the gene. The gene encodes a protein of 686 amino acid long with a molecular weight of 72,000. The gH protein possesses features of a transmembrane glycoprotein. Comparisons of gH protein sequences were made between TNL strain and two published foreign strains, Ka strain and NIA strain, which revealed identities higher than 99%. Northern blot hybridization study revealed that the transcripts of gH is 2.3 kb in size (analog to Ka strain). The RNA is transcribed from 2 to 8 hours after infection.