Effect of Addition n-Hexadecane on Penicillin Fermentation

• Main Authors: Chao-Shien Peng, 彭兆賢
• Other Authors: Te-Ling Chen
• Format: Others
• Language: zh-TW
• Published: 1994
• Online Access: http://ndltd.ncl.edu.tw/handle/98679779761666854399

碩士 === 國立成功大學 === 化學工程研究所 === 82 === Oxygen supply is usually a limiting step in aerobic bio- reaction system. To overcome this drawback, hydrocarbons are added to a fermentatinon medium to increase the oxygen transfer rate. In this study, the fermentation of Penicillium chysogenum was employed to investigate the effect of addition of n-hexade- cane on the mold growth and the production of penicillin. Experimental results showed that the timing of adding n- hexadecane to the fermentation medium would effect cell mor- phology and penicillin productivity. Adding n-hexadecane at growth phase, the mold would exist as filamentous mycelium (not pellet) and the penicillin productivity was low. Adding n- hexadecane at the production phase, the biomass existed as fluffy loose pellets and high penicillin productivity could be obtained. We also found that n-hexadecane had a good effect of anti- foaming. Employing n-hexadecane as the antifoam agent resulted in higher production rate of penicillin than using KM-70.