| Summary: | 碩士 === 國立中山大學 === 海洋生物研究所 === 82 === The present research investigated the absorption and metabolism
of L-[1-13C]methionine in Penaeus monodon to understand the
reasons why the prawn can not efficiently utilize crystalline
amino acids supplemented in diet.Two experiments were
conducted. The first experiment studied the change of L-[1-13C]
methionine concentration in the hemolymph of Penaeus monodon
(20- 30g) within 20 hours after being forced-fed crystalline L-
[1-13C] methionine or cellulose acetate phthalate
microencapsulated L-[1- 13C] methionine(CAP-L-[1-13
C]methionine). The second experiment examined the deposition
and metabolism of L-[1-13C]methionine in the intestine,
Hepatopancreas, muscle, and remaining tissues of Penaeus
monodon (20-30g)within 6 days after being force-fed crystalline
L-[1-13C]methionine or CAP-L-[1-13C]methioine.Penaeus monodon
absorbed efficiently either crystalline-L-[1-13C] methionine or
CAP-L-[1-13C]methionine. No signfifcant difference in the
absorption of CAP-L-[1-13C]methionine or crystalline-L-[1- 13
C]methionine was detected. Almost all the crystalline-L-[1-13
C] and CAP-L-[1-13C]methionine force-fed as absorpted by
Penaeus monodon within the first 2 hours and was transported
promptly to muscle and remaining tissues. Once in muscle,L-[1-13
C] methionine were not metabolized until 10 hours after L-[1-13
C] methionine deposition in Penaeus monodon fed CAP-L-[1-13C]
methionine wasn't significantly higher than that fed
crystalline -L-[1-13C]methionine. The present results do not
support the two assertion that crystalline amino acids are
rapidly absorb and metabolize or the prawn are poor in
absorption of crystalline amino acids.Resutls inclined to
suggest that leaching of crystalline amino acids during feeding
may be the main reason why prawn can not use dietary
crystalline amino acid effectively.
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