The Studies of Toxicity and Detoxification of Cadmium Chloride n Chinese Hamster Ovary Cells

• Main Authors: Tang, Tzung Yin, 唐宗寅
• Other Authors: Huang Hai Mei
• Format: Others
• Language: zh-TW
• Published: 1994
• Online Access: http://ndltd.ncl.edu.tw/handle/29544905875797026159

碩士 === 國立清華大學 === 輻射生物研究所 === 82 === Cisplatin-resistant cells, Pt4 and Pt8 cells, were obtainedom Chinese hamster ovary (CHO-K1) cells after long-term culturecisplatin containing medium. Pt8 cells were about 1.3-foldesistant to cadmium chloride (CdCl2) than CHO-K1 cells. SH content in Pt4 cells was 1.3-fold more than that in4 cells were about 2.9-fold more sensitive to CdCl2after examination with clonogenic survival.20 uM of CdCl2, Pt4 cells were induced aboutmicronucleus than K1 cells were. But Pt8 cellsss micronucleus (about 0.9-fold) than K1 cells were. From nuclear division index, nuclear division in4 cells was inhibited more than that in K1 cells was. Theite results were observed in Pt8 cells (CdCl2 concentration50: K1=20 uM; Pt4=11 uM; Pt8=21.5 uM). In addition,from nucleoid sedimantation assay indicated that the DNAon was significant changed in Pt4 cells after highns of CdCl2 exposure. Also, K1 cells recovered by time dependence after incubatedth fresh medium for 4 or 8 h when treated with CdCl2 for 2 h.Pt4 cells did not recover for 4 or 8 h when treated withfor 2 h. So it seems that Pt4 cells did not recover damagediciently. The detoxfied enzymes, such as catalase,ne S-transferase, and heme oxygenase were nottly different in K1, Pt4 and Pt8 cells. These suggestls were more resistant than K1 cells after exposedmore GSH contents, and Pt4 cells were moreK1 cells after CdCl2 exposure for less reapirmore cadmium accumulation.