| Summary: | 博士 === 國立臺灣大學 === 農業化學系 === 82 === In order to improve the utilization of cellulosic agricultural
wastes, this thesis is concerned with the study of the
feasibility of cellulase production by solid state fermentation
using corn cobs as substrate. In this study, different fungi
and Streptomyces were tested. Results had shown that those
organisms that produced high cellulase activity in liquid state
fermentation were also effective in producing cellulase in
solid state fermentation. So, we decided to select Trichoderma
reesei ATCC 26921 (QM 9414), T. album, Penicillium funiculosum
ATCC 11797, Aspergillus niger ATCC 108764, Streptomyces
aureofaciens ATCC 10762 to study further. Optimal conditions
for producing cellulase in solid fermentation were as follows:
initial moisture 60-65% and initial pH 3.5 for fungal cellulase
production; in initial pH 4.0 -5.0 for Streptomyces cellulase
prduction. The incubation temperature was 25-30℃, for all
tested organisms.Study on the effect of C/N on cellulase
production from tested organisms showed that organic nitrogen
source in C/N 20 can improve cellulase production. For
cellulase production by Trichoderma reesei ATCC 26921 (QM 9414)
using corn cobs as substrate in solid state fermentation, we
gained high cellulase activity by 40-60% ammonium sulfate
precipitation and to purify by Sephadex G100 followed by DEAE
Sephadex A50. Some properties of purified cellulase were
analyzed. The purified cellulase from T. reesei ATCC 26921 (QM
9414) was the most activity at pH 5.0 and at 60℃. The optimal
pH for S. aureofaciens cellulase was 5.0. The optimal
temperature for CMCase and avicelase from S. aureofaciens was
60℃, and the optimal temperature for β-flucosidase and filter
paper activity was 50℃.
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