| Summary: | 碩士 === 淡江大學 === 化學學系 === 82 === Two forms of alpha-galactosidase were demonstrated in squash
(Langaria leucantha) and the two forms were separated by
Sephadex DEAE A-50 column, the form1 can not bind in Sephadex
DEAE A-50 column but the form2 will. It would get over
6000-fold purifica- tion enzyme by ion-exchange chromatography,
Concanavalin A- Sepharose affinity chromatography and
melibiosamine affinity column. The following properties of the
two froms of alpha-galacto- sidase were studied : (1). pH
optimum, (2). pH stability, (3). heat stability, (4). Km, (5).
inhibitions of various inhibitors, (6). the hydration of nature
substrate, and (7). molecular weight. The form2 alpha-
galactosidase also conversed human- blood-group B to O-type.
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