Purification and Characterization Alpha-Galactosidase from Squash (Langaria leucantha)

• Main Authors: Liu Jin-Ying, 劉均瀅
• Other Authors: Su-Fang Chien
• Format: Others
• Language: zh-TW
• Online Access: http://ndltd.ncl.edu.tw/handle/21042708656068578612

碩士 === 淡江大學 === 化學學系 === 82 === Two forms of alpha-galactosidase were demonstrated in squash (Langaria leucantha) and the two forms were separated by Sephadex DEAE A-50 column, the form1 can not bind in Sephadex DEAE A-50 column but the form2 will. It would get over 6000-fold purifica- tion enzyme by ion-exchange chromatography, Concanavalin A- Sepharose affinity chromatography and melibiosamine affinity column. The following properties of the two froms of alpha-galacto- sidase were studied : (1). pH optimum, (2). pH stability, (3). heat stability, (4). Km, (5). inhibitions of various inhibitors, (6). the hydration of nature substrate, and (7). molecular weight. The form2 alpha- galactosidase also conversed human- blood-group B to O-type.