Improvement of Pseudo-Multichannel LCEC Detector And Its Feasibility Study as Intelligent Instrument

• Main Authors: Chung-Yi Li, 黎忠義
• Other Authors: Show-Chuen Chen
• Format: Others
• Language: zh-TW
• Published: 1994
• Online Access: http://ndltd.ncl.edu.tw/handle/59470006408318214851

碩士 === 輔仁大學 === 化學學系 === 83 === The two-way multicomponent analysis for the data generated from HPLC with Diode Array Detection (LC-DAD) is advantageous of peak resolution without the need for training sets. The new Heuristic Evolving Latent Projection (HELP) exempt from the plagued obs- curity of component analysis in the process of data partition. This achievement has no doubt marked a new frontier for chemo- metrics, but remained largely a simulation so far. In this study, implementation of HELP by LC-DAD and LCEC is demonstrated here. With LC-DAD, qualitative results for recon- structed peaks from overlapping peaks with match factor ≧ 999.0 and quantitative results of R=0.99 for calibration curves and a relative standard deviation of 1.3% are attainable. Another attempt of multidetection to generate the data for HELP is the implementation of a pseudo multichannel electrochemical detector (PMD). Several improvements are made to provide sen- sitive data with smaller noise level. Nevertheless, the present PMD though in every respect superior to the its predecessors hardly rivals LC-DAD as far as data quality is concerned. Qualitatively it gives match factor ≧ 994.0, but quantitatively the reconstructed results are far from adequate, with a relative standard deviation of 13%.