| Summary: | 碩士 === 輔仁大學 === 生物學研究所 === 83 === In this study,we used tomato and Fusarium oxysporum to study
the interactions between plants and pathogens. The protein
extracts from tomato cultivar 299 and CF4 can induce
sporulation of Fusarium oxysporum f. sp. lycopersici FO26, but
the protein extract from tomato cultivar 571 does not have this
effect. We further purified the protein which can induce
sporulation of FO26 from tomato cultivar CF4 by using DEAE
chromatography.It can only be found in tomato cultivars CF4 and
299 which were susceptible to FO26 and can't be found in tomato
cultivar 571 which was resistant to FO26.This protein was heat-
stable and it's molecular weight was about 33 KDa.The induction
of sporulation of FO26 by the partial purified plant protein
was influenced by the treatment of divalent cations, various pH
value and light. 0.1mM Mn+2 can inhibit the sporulation of
FO26. The optimal pH for sporulation was 6.5 . Conidial
production increased 4-fold in continueous light than in
darkness. This protein has differential effects of inducing
sporulation in different Fusarium oxysporum races. Based on
these results, we hypothesized that the protein inducing
sporulation was involved in tomato susceptibility toward
pathogen.
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