Studies on difference of shoot tips cultured in vitro in pear cultivars

• Main Authors: Hsiu-Wen Chu, 朱秀雯
• Other Authors: Yau-Shiang Yang
• Format: Others
• Language: zh-TW
• Published: 1995
• Online Access: http://ndltd.ncl.edu.tw/handle/13408086808179643089

碩士 === 國立中興大學 === 園藝學系 === 83 === This study is conducted to investigate the morphology of the apical meristems of six sinensis pears, twenty-five sand pears, two pears, four Taiwan native pears and one hybrid pear. The observation showed that the apical meristems of sinensis pears, China sand pears, pears and evergreen pear were smaller, NiauliA and hibrid pear were bigger. The higher survival percentage were achieved when shoot tip explants of China sand pears, Taiwan native pears and hybrid pear which cultured on the paper bridge medium with 1/2MS containing 1 mg/l BA, 0.5 mg/ l kinetin, 0.2 mg/l IBA and 4 mg/l adenine sulfate for 30 days. A smaller average size of bud were observed in sinensis pears, pears and hybrid pears than other varieties. Additionally, shoot tip explants was found to be the lowest in the average fresh weight in sinensis pears. In the case of shoot proliferation, the multiplication rate of the evergreen pear was the highest among all, followed by NiauliA, NiauliB, NiauliC, hybrid pear, sand pears, and pears is the lowest. Sinensis and some Japanese sand pears could increase the shoot multiplication by using double-phase medium culture. Axillary shoots proliferation was increased when China sand pear explants were cultured on MS with 0-0.1 mg/l of kinetin, although 1-2 mg/l kinetin favored multiplication of explants for Japanese sand pears. Minimal shoot elongation in vitro of pear varieties, which cultured on MS with 0.5-2 mg/l GA were obtained enchancment of shoot elongation. In rooting culture, the bases of the microshoot were dipped in 200 ppm IBA solution by 24 hours resulted in better rooting rate. In the case of poor rooting, shoots were placed on a 1/2MS medium with 2 mg/l NAA and incubated in darkness for 6 days, the propagules transplanted to an auxin-free 1/2MS medium increased rooting rate after 24 days.