Studies on Asymbiotic Germination of Paphiopedilum

• Main Authors: Bih-Yuan LiouHuang, 劉黃碧圓
• Other Authors: Tsai-Yih Wang
• Format: Others
• Language: zh-TW
• Published: 1995
• Online Access: http://ndltd.ncl.edu.tw/handle/52801846692361261235

碩士 === 國立中興大學 === 園藝學系 === 83 === Three species of Paphiopedilum, Paph. purpuratum, Paph. philippinense and Paph. sukhakulii were used on this study. The development of capsules and seeds, germinating seeds to different midia and temperatures and the effects of seedlings to media and the seeds at 4℃ storage were investigated be taken in this experiment. The results are summarized as follows ： 1.The seeds of Paph. purpuratum would turn into yellow 8 months after pollination. Under scanning by electron microscope showed archesporial cells divided one month after pollination, the megaspore developed after 2-4 months, the seed formed after 5 months, and after 11 months, the seeds developed well. The other kind, Paph. philippinense showed 105 days after pollination, the seeds become golden-brown color. 120 days, dark brown, then after 110 days the capsules turned into brown. 2.The seeds were tBih- Yuan LiouHuang media and temperatures showed Paph. purpuratum germinated best in MS medium at 25℃, the percentage of germination was 19.5﹪, the Paph. philippinense did best in RE medium at 25℃, the percentage of germination was 85.5﹪. Paph. sukhakulii did the best in RE medium at 20℃, the percentage of germination was 27.1﹪. The protocorm development of Paph. sukhakulii showed the best result in 1/2MS（A）+B+C+D medium at 20℃, Paph. philippinense did the best in RE medium at 25℃, and Paph. purpuratum in MS medium at 25℃. 3.The seedlings of Paph. sukhakulii grew best in the medium of 1/2MS（A）+B+C+D+20g/l sugar, and Paph. philippinense did the best in the medium 1/2MS（A）+B+C+D+30g/l sugar. 4.The mature seeds of two species were stored 2 months at 4 ℃, showed better germination, above all Paph. purpuratum germinated 2 months earlier.