The roles of topoisomerases in the replication and expression of pseudorrabies virus
碩士 === 國立中興大學 === 獸醫學系 === 83 === Pseudorabies disease is an important infectious disease of swine, and pseudorabies virus is the causative agent. The pseudorabies virus genome is a linear double-stranded DNA(about 145kbp).The...
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ndltd-TW-083NCHU05410102015-10-13T12:53:31Z http://ndltd.ncl.edu.tw/handle/80978567698874234466 The roles of topoisomerases in the replication and expression of pseudorrabies virus 拓樸異構酵素與假性狂犬病毒複製及基因表現的關係 Wang Chi Young 王之仰 碩士 國立中興大學 獸醫學系 83 Pseudorabies disease is an important infectious disease of swine, and pseudorabies virus is the causative agent. The pseudorabies virus genome is a linear double-stranded DNA(about 145kbp).The icosahedral capsid approximately 150-180nm in diameter contains 162 capsomeres , sometimes asymmetric material surrounding the capsid designated as the tegument, and an envelope containing viral glycoprotein spikes on its surface.The pseudorabies virus genome consists of two covalently linked components , designated as unique L(long) and unique S(short), which were bracketed by inverted repeat and terminal repeat.The viral DNA is synthesized by a virus-encoded DNA polymerase and a rolling circle replicating mechanism has been proposed. Our research goal is to elucidate the roles of topoisomerases in the replication and expression of pseudorabies virus. Both type I and II DNA topoisomerases have been found in prokaryotes and eukaryotes. They have important roles in cellular DNA replication and transcription. In animal viruses, topoisomerases also involve in the life cycle of viruses containing closed circular DNA( such as SV 40 virus) as well as viruses possessing double- stranded linear DNA(such as adenovirus and herpesviruses). Our results reveal that type I topoisomerase inhibitor (camptothecin) and type II topoisomerase inhibitors (VP-16 and ellipticine) could inhibit the multiplication of pseudorabies virus. The type I topoisomerase activity is essential for the DNA synthesis of pseudorabies virus.Our findings also suggest that both type I and type II topoisomerases are required for the gene expression of the pseudorabies virus genome. Dr.Wong Min Liang 王孟亮 1995 學位論文 ; thesis 78 zh-TW |
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碩士 === 國立中興大學 === 獸醫學系 === 83 === Pseudorabies disease is an important infectious disease of
swine, and pseudorabies virus is the causative agent. The
pseudorabies virus genome is a linear double-stranded
DNA(about 145kbp).The icosahedral capsid approximately
150-180nm in diameter contains 162 capsomeres ,
sometimes asymmetric material surrounding the capsid
designated as the tegument, and an envelope containing viral
glycoprotein spikes on its surface.The pseudorabies virus
genome consists of two covalently linked components ,
designated as unique L(long) and unique S(short), which
were bracketed by inverted repeat and terminal repeat.The
viral DNA is synthesized by a virus-encoded DNA
polymerase and a rolling circle replicating mechanism
has been proposed. Our research goal is to elucidate the
roles of topoisomerases in the replication and expression of
pseudorabies virus. Both type I and II DNA topoisomerases
have been found in prokaryotes and eukaryotes. They have
important roles in cellular DNA replication and
transcription. In animal viruses, topoisomerases also involve
in the life cycle of viruses containing closed circular DNA(
such as SV 40 virus) as well as viruses possessing
double- stranded linear DNA(such as adenovirus and
herpesviruses). Our results reveal that type I
topoisomerase inhibitor (camptothecin) and type II
topoisomerase inhibitors (VP-16 and ellipticine) could
inhibit the multiplication of pseudorabies virus. The type I
topoisomerase activity is essential for the DNA
synthesis of pseudorabies virus.Our findings also suggest
that both type I and type II topoisomerases are required for
the gene expression of the pseudorabies virus genome.
|
author2 |
Dr.Wong Min Liang |
author_facet |
Dr.Wong Min Liang Wang Chi Young 王之仰 |
author |
Wang Chi Young 王之仰 |
spellingShingle |
Wang Chi Young 王之仰 The roles of topoisomerases in the replication and expression of pseudorrabies virus |
author_sort |
Wang Chi Young |
title |
The roles of topoisomerases in the replication and expression of pseudorrabies virus |
title_short |
The roles of topoisomerases in the replication and expression of pseudorrabies virus |
title_full |
The roles of topoisomerases in the replication and expression of pseudorrabies virus |
title_fullStr |
The roles of topoisomerases in the replication and expression of pseudorrabies virus |
title_full_unstemmed |
The roles of topoisomerases in the replication and expression of pseudorrabies virus |
title_sort |
roles of topoisomerases in the replication and expression of pseudorrabies virus |
publishDate |
1995 |
url |
http://ndltd.ncl.edu.tw/handle/80978567698874234466 |
work_keys_str_mv |
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