| Summary: | 碩士 === 國立成功大學 === 化學工程研究所 === 83 === Hydrolysis of p-nitrophenyl sterate by Candida cylindracea
lipase triacylglycerol acylhydrolases , EC 3.1.1.3）has been
carried out at 35 0C in biphasic aqueous （pH=6.0）organic
systems. By fitting the experimental data to the theoretical
model , the var- iation of kinetic and adsorptive parameters
with the organic sol- vent for the enzyme and substrate was
reached , from which effec- ts of solvent polarity on the
enzymatic reaction were obtained . It was also found that the
less polarity of the solvent is , the less adsorptivity and
reactivity is for the lipase. Moreover , possible explanations
from the changes of enzyme structure were given to relate above
variation with the organic solvents.
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