Production and release of chitinase by recombinant Escherichia coli.

• Main Authors: Ming-Ching Huang, 黃明清
• Other Authors: Professor Chu-Yuan Cheng
• Format: Others
• Language: zh-TW
• Published: 1995
• Online Access: http://ndltd.ncl.edu.tw/handle/95842215056398661626

碩士 === 國立成功大學 === 化學工程研究所 === 83 === The factors that improve recombinant protein excretion efficiency were investigated. The factors discussed in this dissertation include the ratio of carbon to nitrogen source (C/ N ratio), the addition of glycine and EDTA, the addition of alkali metal ions, and the supplement of guanidine-HCl. First, the result was shown that the excretion of model protein- chitinase was more obvious in the C/N ratio=0.1 than the other following C/N ratios. Second, it was indicated that the excretion ofchitinase increased with glycine and EDTA concentration. Besides, addition time of glycine and EDTA also influences the release of protein. Next, it was found that supplement of alkali metal ions would improve chitinase produ- ction and excretion, especially Na+. Although addition of guan- idine-HCl in growing cell culture broth, protein was denatured besides cell growth limited. It was thought that the improve- ment of chitinaseexcretion into culture medium by the factors mentioned abovewere primary due to cell outer membrane perme- ability.